Redistribution of intracellular Ca2+ stores during phagocytosis in human neutrophils

Olle Stendahl, Karl Heinz Krause, Joachim Krischer, Petra Jerström, Jean Marc Theler, Robert A. Clark, Jean Louis Carpentier, Daniel P. Lew

Research output: Contribution to journalArticlepeer-review

126 Scopus citations

Abstract

Subcellular gradients of cytosolic free Ca2+ concentration, [Ca2+]i, are thought to be critical for the localization of functional responses within a cell. A potential but previously unexplored mechanism for the generation of gradients of [Ca2+]i is the accumulation of Ca2+ stores at the site of Ca2+ action. The distribution of the Ca2+ store markers Ca2+-dependent adenosine triphosphatase and calreticulin was investigated in resting and phagocytosing human neutrophils. Both proteins showed an evenly distributed fine granular pattern in nonphagocytosing cells, but became markedly concentrated in the filamentous actin-rich cytoplasmic area around the ingested particle during phagocytosis. This redistribution began at early stages of phagocytosis and did not depend on an increase in [Ca2+]i. Thus, accumulation of Ca2+ stores in a restricted area of the cell may contribute to the generation of localized increases in [Ca2+]i.

Original languageEnglish (US)
Pages (from-to)1439-1441
Number of pages3
JournalScience
Volume265
Issue number5177
DOIs
StatePublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • General

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