Red blood cells are a sink for interleukin 8, a leukocyte chemotaxin

Walter C. Darbonne, Glenn C. Rice, Margorie A. Mohler, Ted Apple, Caroline A. Hébert, Anthony J. Valente, Joffre B. Baker

Research output: Contribution to journalArticlepeer-review

347 Scopus citations


IL-8 (also known as neutrophil-activating peptide 1) is recognized as a potent effector of neutrophil functions. Several different cell types that contact blood, namely T lymphocytes, monocytes, and endothelial cells, secrete this polypeptide following stimulation by cytokines, or lipopolysaccharide. Here we show that when IL-8 is added to blood it rapidly partitions from the plasma fluid to the blood cells and that erythrocytes account for the vast majority of this binding. Analysis of 125I-IL-8 binding ([ala-IL-8]77 form) to human red cells indicates a single, 5 nM Kd affinity class of binding sites, present at ≈ 2,000 per red cell representing ∼ 15 nmol of red cell IL-8 binding sites per liter of blood. These sites are protease sensitive. Their binding of IL-8 is rapidly reversible and does not result in receptor internalization, although bound IL-8 is resistant to extraction by pH 3 buffer at 5°C. 125I-IL-8 binding to red cells was not inhibited by epidermal growth factor or interleukin 1, but was inhibited by monocyte chemotactic peptide-1, which is not a neutrophil chemotaxin, but is a member of the same family of polypeptides as IL-8. FACS® analysis of IL-8-mediated mobilization of Ca2+ in neutrophils indicates that the IL-8 bound to red cells is incapable of stimulating neutrophils. Thus, red cell absorption of IL-8 may function to limit stimulation of leukocytes by IL-8 released into blood.

Original languageEnglish (US)
Pages (from-to)1362-1369
Number of pages8
JournalJournal of Clinical Investigation
Issue number4
StatePublished - 1991
Externally publishedYes


  • Equilibrium dialysis
  • Erythrocyte
  • Fluorescence activated cell sorter analysis
  • Monocyte chemotactic peptide 1
  • Neutrophil
  • Neutrophil-activating peptide 1

ASJC Scopus subject areas

  • Medicine(all)


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