Recovery from checkpoint-mediated arrest after repair of a double-strand break requires Srs2 helicase

Moreshwar B. Vaze, Achille Pellicioli, Sang Lee, Grzegorz Ira, Giordano Liberi, Ayelet Arbel-Eden, Marco Foiani, James E. Haber

Research output: Contribution to journalArticle

236 Citations (Scopus)

Abstract

In Saccharomyces strains in which homologous recombination is delayed sufficiently to activate the DNA damage checkpoint, Rad53p checkpoint kinase activity appears 1 hr after DSB induction and disappears soon after completion of repair. Cells lacking Srs2p helicase fail to recover even though they apparently complete DNA repair; Rad53p kinase remains activated. srs2Δ cells also fail to adapt when DSB repair is prevented. The recovery defect of srs2Δ is suppressed in mec1Δ strains lacking the checkpoint or when DSB repair occurs before checkpoint activation. Permanent preanaphase arrest of srs2Δ cells is reversed by the addition of caffeine after cells have arrested. Thus, in addition to its roles in recombination, Srs2p appears to be needed to turn off the DNA damage checkpoint.

Original languageEnglish (US)
Pages (from-to)373-385
Number of pages13
JournalMolecular Cell
Volume10
Issue number2
DOIs
StatePublished - Aug 2002
Externally publishedYes

Fingerprint

DNA Damage
Phosphotransferases
Saccharomyces
Homologous Recombination
Caffeine
DNA Repair
Genetic Recombination
1,2-di-(4-sulfamidophenyl)-4-butylpyrazolidine-3,5-dione

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Recovery from checkpoint-mediated arrest after repair of a double-strand break requires Srs2 helicase. / Vaze, Moreshwar B.; Pellicioli, Achille; Lee, Sang; Ira, Grzegorz; Liberi, Giordano; Arbel-Eden, Ayelet; Foiani, Marco; Haber, James E.

In: Molecular Cell, Vol. 10, No. 2, 08.2002, p. 373-385.

Research output: Contribution to journalArticle

Vaze, MB, Pellicioli, A, Lee, S, Ira, G, Liberi, G, Arbel-Eden, A, Foiani, M & Haber, JE 2002, 'Recovery from checkpoint-mediated arrest after repair of a double-strand break requires Srs2 helicase', Molecular Cell, vol. 10, no. 2, pp. 373-385. https://doi.org/10.1016/S1097-2765(02)00593-2
Vaze, Moreshwar B. ; Pellicioli, Achille ; Lee, Sang ; Ira, Grzegorz ; Liberi, Giordano ; Arbel-Eden, Ayelet ; Foiani, Marco ; Haber, James E. / Recovery from checkpoint-mediated arrest after repair of a double-strand break requires Srs2 helicase. In: Molecular Cell. 2002 ; Vol. 10, No. 2. pp. 373-385.
@article{8727d0f93c334b4d9cd5a2acf84b4c33,
title = "Recovery from checkpoint-mediated arrest after repair of a double-strand break requires Srs2 helicase",
abstract = "In Saccharomyces strains in which homologous recombination is delayed sufficiently to activate the DNA damage checkpoint, Rad53p checkpoint kinase activity appears 1 hr after DSB induction and disappears soon after completion of repair. Cells lacking Srs2p helicase fail to recover even though they apparently complete DNA repair; Rad53p kinase remains activated. srs2Δ cells also fail to adapt when DSB repair is prevented. The recovery defect of srs2Δ is suppressed in mec1Δ strains lacking the checkpoint or when DSB repair occurs before checkpoint activation. Permanent preanaphase arrest of srs2Δ cells is reversed by the addition of caffeine after cells have arrested. Thus, in addition to its roles in recombination, Srs2p appears to be needed to turn off the DNA damage checkpoint.",
author = "Vaze, {Moreshwar B.} and Achille Pellicioli and Sang Lee and Grzegorz Ira and Giordano Liberi and Ayelet Arbel-Eden and Marco Foiani and Haber, {James E.}",
year = "2002",
month = "8",
doi = "10.1016/S1097-2765(02)00593-2",
language = "English (US)",
volume = "10",
pages = "373--385",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "2",

}

TY - JOUR

T1 - Recovery from checkpoint-mediated arrest after repair of a double-strand break requires Srs2 helicase

AU - Vaze, Moreshwar B.

AU - Pellicioli, Achille

AU - Lee, Sang

AU - Ira, Grzegorz

AU - Liberi, Giordano

AU - Arbel-Eden, Ayelet

AU - Foiani, Marco

AU - Haber, James E.

PY - 2002/8

Y1 - 2002/8

N2 - In Saccharomyces strains in which homologous recombination is delayed sufficiently to activate the DNA damage checkpoint, Rad53p checkpoint kinase activity appears 1 hr after DSB induction and disappears soon after completion of repair. Cells lacking Srs2p helicase fail to recover even though they apparently complete DNA repair; Rad53p kinase remains activated. srs2Δ cells also fail to adapt when DSB repair is prevented. The recovery defect of srs2Δ is suppressed in mec1Δ strains lacking the checkpoint or when DSB repair occurs before checkpoint activation. Permanent preanaphase arrest of srs2Δ cells is reversed by the addition of caffeine after cells have arrested. Thus, in addition to its roles in recombination, Srs2p appears to be needed to turn off the DNA damage checkpoint.

AB - In Saccharomyces strains in which homologous recombination is delayed sufficiently to activate the DNA damage checkpoint, Rad53p checkpoint kinase activity appears 1 hr after DSB induction and disappears soon after completion of repair. Cells lacking Srs2p helicase fail to recover even though they apparently complete DNA repair; Rad53p kinase remains activated. srs2Δ cells also fail to adapt when DSB repair is prevented. The recovery defect of srs2Δ is suppressed in mec1Δ strains lacking the checkpoint or when DSB repair occurs before checkpoint activation. Permanent preanaphase arrest of srs2Δ cells is reversed by the addition of caffeine after cells have arrested. Thus, in addition to its roles in recombination, Srs2p appears to be needed to turn off the DNA damage checkpoint.

UR - http://www.scopus.com/inward/record.url?scp=0036671706&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036671706&partnerID=8YFLogxK

U2 - 10.1016/S1097-2765(02)00593-2

DO - 10.1016/S1097-2765(02)00593-2

M3 - Article

VL - 10

SP - 373

EP - 385

JO - Molecular Cell

JF - Molecular Cell

SN - 1097-2765

IS - 2

ER -