Recording Leukocyte Rolling and Adhesion on Meningeal Vessels by Intravital Microscopy

Aline Silva de Miranda, Thiago Macedo Cordeiro, Milene Alvarenga Rachid, Antônio Lúcio Teixeira

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Leukocyte infiltration in the central nervous system (CNS) has been implicated in several neuroinflammatory diseases, being an important step in the development of inflammatory response in the brain. The use of intravital microscopy technique allows direct in vivo assessment of leukocyte rolling and adhesion on cortical meningeal vessels. The current review highlights the use of intravital microscopy and its association with neuroinflammatory parameters in different models of experimental brain diseases such as HSV-1 encephalitis, dengue encephalitis, cerebral malaria, and hepatic encephalopathy. Herein, we also describe a detailed protocol of intravital microscopy of meningeal vessels, its advantages and major concerns.

Original languageEnglish (US)
Title of host publicationNeuromethods
PublisherHumana Press
Pages315-327
Number of pages13
DOIs
StatePublished - 2019
Externally publishedYes

Publication series

NameNeuromethods
Volume142
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • Brain
  • Chemokines
  • Intravital microscopy
  • Leukocyte adhesion
  • Leukocyte rolling
  • Mice
  • Pia mater vessels

ASJC Scopus subject areas

  • General Neuroscience
  • General Biochemistry, Genetics and Molecular Biology
  • General Pharmacology, Toxicology and Pharmaceutics
  • Psychiatry and Mental health

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