Reconstituting the Key Steps of the DNA Double-Strand Break Repair In Vitro

Matthew J. Rossi, Dmitry V. Bugreev, Olga M. Mazina, Alexander V. Mazin

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Double-stranded DNA breaks (DSB), the most harmful type of DNA lesions, cause cell death and genome instability. Homologous recombination repairs DSB using homologous DNA sequences as templates. Here we describe a set of reactions that lead to reconstitution of the double-stranded DNA break repair process in vitro employing purified human homologous recombination proteins and DNA polymerase η. Reconstitution of critical steps of DSB repair in vitro may help to better understand the mechanisms of recombinational DNA repair and the role of various human homologous recombination proteins in this process.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Number of pages14
StatePublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • D-loops
  • DNA strand exchange
  • Holliday junction
  • Homologous recombination
  • branch migration
  • joint molecules

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology


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