Recombination can lead to spurious results in retroviral transduction with dually fluorescent reporter genes

Daniel J. Salamango, David A. Evans, Mariju F. Baluyot, Jackson N. Furlong, Marc C. Johnson

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Fluorescent proteins are routinely employed as reporters in retroviral vectors. Here, we demonstrate that transduction with retroviral vectors carrying a tandem-dimer Tomato (TdTom) reporter produces two distinct fluorescent cell populations following template jumping due to a single nucleotide polymorphism between the first and second Tomato genes. Template jumping also occurs between repeated sequences in the Tomato and green fluorescent protein (GFP) genes. Thus, proper interpretation of the fluorescence intensity of transduced cells requires caution.

Original languageEnglish (US)
Pages (from-to)13900-13903
Number of pages4
JournalJournal of virology
Volume87
Issue number24
DOIs
StatePublished - Dec 2013
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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