Receptor specificity of the fibroblast growth factor family

David M. Ornitz, Jingsong Xu, Jennifer S. Colvin, Donald G. McEwen, Craig A. MacArthur, François Coulier, Guangxia Gao, Mitchell Goldfarb

Research output: Contribution to journalArticle

1338 Scopus citations

Abstract

Fibroblast growth factors (FGFs) are essential molecules for mammalian development. The nine known FGF ligands and the four signaling FGF receptors (and their alternatively spliced variants) are expressed in specific spatial and temporal patterns. The activity of this signaling pathway is regulated by ligand binding specificity, heparan sulfate proteoglycans, and the differential signaling capacity of individual FGF receptors. To determine potentially relevant ligand-receptor pairs we have engineered mitogenically responsive cell lines expressing the major splice variants of all the known FGF receptors. We have assayed the mitogenic activity of the nine known FGF ligands on these cell lines. These studies demonstrate that FGF 1 is the only FGF that can activate all FGF receptor splice variants. Using FGF 1 as an internal standard we have determined the relative activity of all the other members of the FGF family. These data should serve as a biochemical foundation for determining developmental, physiological, and pathophysiological processes that involve FGF signaling pathways.

Original languageEnglish (US)
Pages (from-to)15292-15297
Number of pages6
JournalJournal of Biological Chemistry
Volume271
Issue number25
DOIs
StatePublished - 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Receptor specificity of the fibroblast growth factor family'. Together they form a unique fingerprint.

Cite this