Receptor binding of PDGF-AA and PDGF-BB, and the modulation of PDGF receptors by TGF-β, in human periodontal ligament cells

T. W. Oates, K. N. Kose, J. F. Xie, D. T. Graves, J. M. Collins, David L Cochran

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The growth factors PDGF-AA and PDGF-BB have previously been shown to be potent mitogens for human periodontal ligament (hPDL) cells in vitro. Additionally, the mitogenic response to PDGF-AA has been shown to be specifically inhibited by TGF-β. The purpose of the present investigation was to examine the binding of PDGF-AA and PDGF-BB, and the modulation of PDGF binding by TCF-β, in hPDL cells. Scatchard analysis identified an average of 32,000 PDGF-AA high-affinity binding sites per cell with a dissociation constant (K(d)) of 0.66 nM and an average of 36,000 PDGF-BB binding sites per cell with a dissociation constant (k(d)) of 0.44 nM. After treatment with TGF-β, the receptor number for PDGF-AA was found to specifically decrease by approximately 50%, with no change in binding affinity. This reduced number of binding sites was shown to correlate with both a decrease in levels of receptor tyrosine phosphorylation and a decreased number of α receptor subunits. Northern blot analysis identified the TGF-β-mediated decrease in PDGF α receptor subunit mRNA levels. PDGF-BB showed little change in the number of binding sites or in the binding affinity with TGF-β treatment, and the data were consistent with an increase in the number of β receptor subunits. These results demonstrate nearly equivalent numbers of receptors for both PDGF-AA and PDGF-BB in hPDL cells. Also, modulation of PDGF binding, by TFG-β, was shown to result in a reduced number of α receptor subunits with an increase in the number of β receptor subunits.

Original languageEnglish (US)
Pages (from-to)359-366
Number of pages8
JournalJournal of Cellular Physiology
Volume162
Issue number3
DOIs
StatePublished - Mar 1995

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Platelet-Derived Growth Factor Receptors
Periodontal Ligament
Ligaments
Modulation
Binding Sites
Phosphorylation
Mitogens
Northern Blotting
platelet-derived growth factor A
platelet-derived growth factor BB
Intercellular Signaling Peptides and Proteins
Messenger RNA

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Receptor binding of PDGF-AA and PDGF-BB, and the modulation of PDGF receptors by TGF-β, in human periodontal ligament cells. / Oates, T. W.; Kose, K. N.; Xie, J. F.; Graves, D. T.; Collins, J. M.; Cochran, David L.

In: Journal of Cellular Physiology, Vol. 162, No. 3, 03.1995, p. 359-366.

Research output: Contribution to journalArticle

Oates, T. W. ; Kose, K. N. ; Xie, J. F. ; Graves, D. T. ; Collins, J. M. ; Cochran, David L. / Receptor binding of PDGF-AA and PDGF-BB, and the modulation of PDGF receptors by TGF-β, in human periodontal ligament cells. In: Journal of Cellular Physiology. 1995 ; Vol. 162, No. 3. pp. 359-366.
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