Reactivation of the Bacillus subtilis anti-σ(B) antagonist, RsbV, by stress- or starvation-induced phosphatase activities

Uwe Voelker, Andrea Voelker, William G. Haldenwang

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

σ(B) is a secondary σ factor that controls the general stress regulon in Bacillus subtilis. The regulon is activated when σ(B) is released from a complex with an anti-σ(B) protein (RsbW) and becomes free to associate with RNA polymerase. Two separate mechanisms cause σ(B) release: an ATP- responsive mechanism that correlates with nutritional stress and an ATP- independent mechanism that responds to environmental insult (e.g., heat shock and ethanol treatment). ATP levels are thought to directly affect RsbW's binding preference. Low levels of ATP cause RsbW to release σ(B) and bind to an alternative protein (RsbV), while high levels of ATP favor RsbW-σ(B) complex formation and inactivation of RsbV by an RsbW-dependent phosphorylation. During growth, most of the RsbV is phosphorylated (RsbV-P) and inactive. Environmental stress induces the release of σ(B) and the formation of the RsbW-RsbV complex, regardless of ATP levels. This pathway requires the products of additional genes encoded within the eight-gene operon (sigB) that includes the genes for σ(B), RsbW, and RsbV. By using isoelectric focusing techniques to distinguish RsbV from RsbV-P and chloramphenicol treatment or pulse-chase labeling to identify preexisting RsbV-P, we have now determined that stress induces the dephosphorylation of RsbV-P to reactivate RsbV. RsbV-P was also found to be dephosphorylated upon a drop in intracellular ATP levels. The stress-dependent and ATP-responsive dephosphorylations of RsbV-P differed in their requirements for the products of the first four genes (rsbR, -S, -T, and -U) of the sigB operon. Both dephosphorylation reactions required at least one of the genes included in a deletion that removed rsbR, -S, and -T; however, only an environmental insult required RsbU to reactivate RsbV.

Original languageEnglish (US)
Pages (from-to)5456-5463
Number of pages8
JournalJournal of bacteriology
Volume178
Issue number18
DOIs
StatePublished - 1996

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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