Reactions of benzo[a]pyrene diol-epoxides with DNA and nucleosomes in aqueous solutions

A. Kootstra, B. L. Haas, T. J. Slaga

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

The rate of solvolysis of benzo[a]pyrene diol-epoxide in aqueous solutions can be followed by fluorescence spectroscopy. When DNA was present the rat of breakdown of benzo[a]pyrene diol-epoxide was substantially enhanced, while at the same time fluorescence intensity was decreased. This decrease, however, was due to noncovalently bound tetraols and does not seem to be a function of the covalent adducts formed. Nucleosomal core particles, reacted under identical conditions, showed very little quenching of the pyrene-like chromophore. When increasing amounts of cysteine were present the covalent binding could be prevented in both free DNA and nucleosomal DNA. Analysis of the distribution of the carcinogen to nucleosomal DNA showed that the covalently bound carcinogen was located at or within 10 bases of the 5′-OH region of the nucleosomal DNA.

Original languageEnglish (US)
Pages (from-to)1432-1438
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume94
Issue number4
DOIs
StatePublished - Jun 30 1980
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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