Reaction of 5 s RNA with a radioactive carbodiimide

John C. Lee; Vernon M. Ingram

doi:10.1016/0022-2836(69)90286-1

Reaction of 5 s RNA with a radioactive carbodiimide

John C. Lee, Vernon M. Ingram

Biochemistry & Structural Biology

Research output: Contribution to journal › Article

23 Scopus citations

Abstract

Escherichia coli 5 s RNA was reacted with 1-cyclohexyl-3-(2-morpholinyl-(4)-ethyl) carbodiimide [¹⁴C]methoiodide in the presence of magnesium ion. The RNA molecule seems highly compact in that most of its bases are inaccessible to the carbodiimide. However, the uridine residues in two sequences, UpApGp and ApCpCpCpCpApUpGp, are more exposed and available to reaction with the reagent, which throws light on the conformation of the RNA molecule.

Original language	English (US)
Pages (from-to)	431-441
Number of pages	11
Journal	Journal of Molecular Biology
Volume	41
Issue number	3
DOIs	https://doi.org/10.1016/0022-2836(69)90286-1
Publication status	Published - May 14 1969

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ASJC Scopus subject areas

Structural Biology
Molecular Biology

Cite this

Lee, J. C., & Ingram, V. M. (1969). Reaction of 5 s RNA with a radioactive carbodiimide. Journal of Molecular Biology, 41(3), 431-441. https://doi.org/10.1016/0022-2836(69)90286-1

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AB - Escherichia coli 5 s RNA was reacted with 1-cyclohexyl-3-(2-morpholinyl-(4)-ethyl) carbodiimide [14C]methoiodide in the presence of magnesium ion. The RNA molecule seems highly compact in that most of its bases are inaccessible to the carbodiimide. However, the uridine residues in two sequences, UpApGp and ApCpCpCpCpApUpGp, are more exposed and available to reaction with the reagent, which throws light on the conformation of the RNA molecule.

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10.1016/0022-2836(69)90286-1