Reaction of 5 s RNA with a radioactive carbodiimide

John C. Lee, Vernon M. Ingram

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Escherichia coli 5 s RNA was reacted with 1-cyclohexyl-3-(2-morpholinyl-(4)-ethyl) carbodiimide [14C]methoiodide in the presence of magnesium ion. The RNA molecule seems highly compact in that most of its bases are inaccessible to the carbodiimide. However, the uridine residues in two sequences, UpApGp and ApCpCpCpCpApUpGp, are more exposed and available to reaction with the reagent, which throws light on the conformation of the RNA molecule.

Original languageEnglish (US)
Pages (from-to)431-441
Number of pages11
JournalJournal of Molecular Biology
Volume41
Issue number3
StatePublished - May 14 1969
Externally publishedYes

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Carbodiimides
RNA
Nucleic Acid Conformation
Uridine
Magnesium
Ions
Escherichia coli
1-cyclohexyl-3-(2-(4-morpholinyl)ethyl)carbodiimide

ASJC Scopus subject areas

  • Virology

Cite this

Lee, J. C., & Ingram, V. M. (1969). Reaction of 5 s RNA with a radioactive carbodiimide. Journal of Molecular Biology, 41(3), 431-441.

Reaction of 5 s RNA with a radioactive carbodiimide. / Lee, John C.; Ingram, Vernon M.

In: Journal of Molecular Biology, Vol. 41, No. 3, 14.05.1969, p. 431-441.

Research output: Contribution to journalArticle

Lee, JC & Ingram, VM 1969, 'Reaction of 5 s RNA with a radioactive carbodiimide', Journal of Molecular Biology, vol. 41, no. 3, pp. 431-441.
Lee, John C. ; Ingram, Vernon M. / Reaction of 5 s RNA with a radioactive carbodiimide. In: Journal of Molecular Biology. 1969 ; Vol. 41, No. 3. pp. 431-441.
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