Reaction of 5 s RNA with a radioactive carbodiimide

John C. Lee; Vernon M. Ingram

doi:10.1016/0022-2836(69)90286-1

Reaction of 5 s RNA with a radioactive carbodiimide

John C. Lee, Vernon M. Ingram

Biochemistry & Structural Biology

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Escherichia coli 5 s RNA was reacted with 1-cyclohexyl-3-(2-morpholinyl-(4)-ethyl) carbodiimide [¹⁴C]methoiodide in the presence of magnesium ion. The RNA molecule seems highly compact in that most of its bases are inaccessible to the carbodiimide. However, the uridine residues in two sequences, UpApGp and ApCpCpCpCpApUpGp, are more exposed and available to reaction with the reagent, which throws light on the conformation of the RNA molecule.

Original language	English (US)
Pages (from-to)	431-441
Number of pages	11
Journal	Journal of Molecular Biology
Volume	41
Issue number	3
DOIs	https://doi.org/10.1016/0022-2836(69)90286-1
State	Published - May 14 1969

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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title = "Reaction of 5 s RNA with a radioactive carbodiimide",

abstract = "Escherichia coli 5 s RNA was reacted with 1-cyclohexyl-3-(2-morpholinyl-(4)-ethyl) carbodiimide [14C]methoiodide in the presence of magnesium ion. The RNA molecule seems highly compact in that most of its bases are inaccessible to the carbodiimide. However, the uridine residues in two sequences, UpApGp and ApCpCpCpCpApUpGp, are more exposed and available to reaction with the reagent, which throws light on the conformation of the RNA molecule.",

author = "Lee, {John C.} and Ingram, {Vernon M.}",

note = "Funding Information: We acknowledget he valuable technical assistanceo f Mm Leslie D. Schroedera nd Mrs Wendy P. Kennedy and the help of Dr S. W. Brostoff in the initial preparation of the radioactive carbodiimide. We also wish to thank Picker Nuclear for the loan of a LCS-1000 Nucleic Acid Analyzer. This investigation was supported by a grant no. AM08390 from the U.S. Public Health Service.",

year = "1969",

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doi = "10.1016/0022-2836(69)90286-1",

language = "English (US)",

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T1 - Reaction of 5 s RNA with a radioactive carbodiimide

AU - Lee, John C.

AU - Ingram, Vernon M.

N1 - Funding Information: We acknowledget he valuable technical assistanceo f Mm Leslie D. Schroedera nd Mrs Wendy P. Kennedy and the help of Dr S. W. Brostoff in the initial preparation of the radioactive carbodiimide. We also wish to thank Picker Nuclear for the loan of a LCS-1000 Nucleic Acid Analyzer. This investigation was supported by a grant no. AM08390 from the U.S. Public Health Service.

PY - 1969/5/14

Y1 - 1969/5/14

N2 - Escherichia coli 5 s RNA was reacted with 1-cyclohexyl-3-(2-morpholinyl-(4)-ethyl) carbodiimide [14C]methoiodide in the presence of magnesium ion. The RNA molecule seems highly compact in that most of its bases are inaccessible to the carbodiimide. However, the uridine residues in two sequences, UpApGp and ApCpCpCpCpApUpGp, are more exposed and available to reaction with the reagent, which throws light on the conformation of the RNA molecule.

AB - Escherichia coli 5 s RNA was reacted with 1-cyclohexyl-3-(2-morpholinyl-(4)-ethyl) carbodiimide [14C]methoiodide in the presence of magnesium ion. The RNA molecule seems highly compact in that most of its bases are inaccessible to the carbodiimide. However, the uridine residues in two sequences, UpApGp and ApCpCpCpCpApUpGp, are more exposed and available to reaction with the reagent, which throws light on the conformation of the RNA molecule.

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JF - Journal of Molecular Biology

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Reaction of 5 s RNA with a radioactive carbodiimide

Abstract

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