Reaction of 5 s RNA with a radioactive carbodiimide

John C. Lee, Vernon M. Ingram

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Escherichia coli 5 s RNA was reacted with 1-cyclohexyl-3-(2-morpholinyl-(4)-ethyl) carbodiimide [14C]methoiodide in the presence of magnesium ion. The RNA molecule seems highly compact in that most of its bases are inaccessible to the carbodiimide. However, the uridine residues in two sequences, UpApGp and ApCpCpCpCpApUpGp, are more exposed and available to reaction with the reagent, which throws light on the conformation of the RNA molecule.

Original languageEnglish (US)
Pages (from-to)431-441
Number of pages11
JournalJournal of Molecular Biology
Issue number3
StatePublished - May 14 1969

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology


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