Abstract
Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA microarray. Comparison of gene expression levels arising from cohybridized samples is achieved by taking ratios of average expression levels for individual genes. A novel method of image segmentation is provided to identify cDNA target sites and a hypothesis test and confidence interval is developed to quantify the significance of observed differences in expression ratios. In particular, the probability density of the ratio and the maximum-likelihood estimator for the distribution are derived, and an iterative procedure for signal calibration is developed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 364-374 |
| Number of pages | 11 |
| Journal | Journal of biomedical optics |
| Volume | 2 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1997 |
| Externally published | Yes |
Keywords
- CDNA
- Gene expression
- Image segmentation
- Mann-Whitney target detection
- Microarray
- Ratio confidence interval
- Ratio density
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Biomedical Engineering
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