Ratio-based decisions and the quantitative analysis of cDNA microarray images

Yidong Chen, Edward R. Dougherty, Michael L. Bittner

Research output: Contribution to journalArticlepeer-review

708 Scopus citations

Abstract

Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA microarray. Comparison of gene expression levels arising from cohybridized samples is achieved by taking ratios of average expression levels for individual genes. A novel method of image segmentation is provided to identify cDNA target sites and a hypothesis test and confidence interval is developed to quantify the significance of observed differences in expression ratios. In particular, the probability density of the ratio and the maximum-likelihood estimator for the distribution are derived, and an iterative procedure for signal calibration is developed.

Original languageEnglish (US)
Pages (from-to)364-374
Number of pages11
JournalJournal of biomedical optics
Volume2
Issue number4
DOIs
StatePublished - 1997
Externally publishedYes

Keywords

  • CDNA
  • Gene expression
  • Image segmentation
  • Mann-Whitney target detection
  • Microarray
  • Ratio confidence interval
  • Ratio density

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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