TY - JOUR
T1 - Rat Pit-1 Stimulates Transcription in Vitro by Influencing Preinitiation Complex Assembly
AU - Sharp, Z. D.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1995/1/5
Y1 - 1995/1/5
N2 - The anterior pituitary-specific transcription factor, Pit-1, activates prolactin, growth hormone, TSHβ, growth hormone receptor genes and autoregulates the pit-1 gene. Its mechanism of transcription activation is unknown. Using immobilized DNA templates and order-of-addition transcription assays, it is shown that Pit-1 is required during pre-initiation complex assembly to activate the prolactin gene in vitro. Using prolactin promoters containing point mutations in the distal TATA box, it is also demonstrated that Pit-1 activation in vitro is not mediated simply by repressing the upstream, alternative promoter. Experiments show that a preformed class II pre-initiation complex is refractory to Pit-1 influence. The data indicate that Pit-1, and perhaps other members of the POU-protein family, activate transcription by influencing the type pre-initiation complex assembled on target promoters.
AB - The anterior pituitary-specific transcription factor, Pit-1, activates prolactin, growth hormone, TSHβ, growth hormone receptor genes and autoregulates the pit-1 gene. Its mechanism of transcription activation is unknown. Using immobilized DNA templates and order-of-addition transcription assays, it is shown that Pit-1 is required during pre-initiation complex assembly to activate the prolactin gene in vitro. Using prolactin promoters containing point mutations in the distal TATA box, it is also demonstrated that Pit-1 activation in vitro is not mediated simply by repressing the upstream, alternative promoter. Experiments show that a preformed class II pre-initiation complex is refractory to Pit-1 influence. The data indicate that Pit-1, and perhaps other members of the POU-protein family, activate transcription by influencing the type pre-initiation complex assembled on target promoters.
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U2 - 10.1006/bbrc.1995.1006
DO - 10.1006/bbrc.1995.1006
M3 - Article
C2 - 7529501
AN - SCOPUS:0028843135
VL - 206
SP - 40
EP - 45
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -