Rapid Identification of Rectal Multidrug-resistant Escherichia coli before Transrectal Prostate Biopsy

Veronika L. Tchesnokova, Linda L. Ottley, Kyoko Sakamoto, Joshua Fierer, Evgeni Sokurenko, Michael A. Liss

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Objective To develop and evaluate a rapid multiplex-quantitative polymerase chain reaction (qPCR) to identify fecal carriers of multidrug-resistant extraintestinal pathogenic Escherichia coli (MDR-ExPEC) clonal groups. Methods Men presenting for transrectal prostate biopsy (TPB) at the San Diego Veterans Affairs Medical Center underwent rectal culture immediately before TPB. Rectal swabs were streaked onto ciprofloxacin-supplemented (4 mg/L) MacConkey agar plates, identified, and susceptibility tested. The same swab was sent to the University of Washington for qPCR test (EST200) targeting 2 major MDR-ExPEC clonal groups - ST131 and ST69 - that combined were expected to represent majority of fluoroquinolone (FQ)- and trimethoprim-sulfamethoxazole-resistant E coli. We calculate test characteristics including the area under the receiver operative curve (AUC). Results We enrolled 104 men from 11/5/2013 to 6/10/2014. FQ-resistant E coli were cultured from 19.2% (20/104) of rectal swabs, and 26% (27/104) of all swabs were positive for EST200 by PCR. The test characteristics comparing the EST200 to the culture-based detection of FQ resistance were 75%, 86%, 94%, and 56%, respectively. The AUC was 0.84 for the EST200 to detect FQ resistance before TPB. Conclusion Compared to the reference standard rectal culture, EST200 was able to detect majority of FQ-resistant E coli on rectal swabs before prostate biopsy.

Original languageEnglish (US)
Pages (from-to)1200-1205
Number of pages6
Issue number6
StatePublished - Dec 1 2015

ASJC Scopus subject areas

  • Urology


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