TY - JOUR
T1 - Rapid determination of macrolide and lincosamide resistance in group B streptococcus isolated from vaginal-rectal swabs
AU - Dela Cruz, Wilfred P.
AU - Richardson, Joann Y.
AU - Broestler, Judith M.
AU - Thornton, Jennifer A.
AU - Danaher, Patrick J.
PY - 2007
Y1 - 2007
N2 - Objective. Our objective was to assess the ability of real-time PCR to predict in vitro resistance in isolates of group B streptococcus (GBS). Methods. The first real-time PCR assays for the genes known to confer resistance to erythromycin and clindamycin in GBS were developed. Three hundred and forty clinical GBS isolates were assessed with these assays and compared with conventional disk diffusion. Results. The presence of an erythromycin ribosome methylation gene (ermB or ermTR variant A) predicted in vitro constitutive or inducible resistance to clindamycin with a sensitivity of 93% (95% CI 86%-97%), specificity of 90% (95% CI 85%-93%), positive predictive value of 76% (95% CI 67%-84%), and negative predictive value of 97% (95% CI 94%-99%). Conclusion. This rapid and simple assay can predict in vitro susceptibility to clindamycin within two hours of isolation as opposed to 18-24 hours via disk diffusion. The assay might also be used to screen large numbers of batched isolates to establish the prevalence of resistance in a given area.
AB - Objective. Our objective was to assess the ability of real-time PCR to predict in vitro resistance in isolates of group B streptococcus (GBS). Methods. The first real-time PCR assays for the genes known to confer resistance to erythromycin and clindamycin in GBS were developed. Three hundred and forty clinical GBS isolates were assessed with these assays and compared with conventional disk diffusion. Results. The presence of an erythromycin ribosome methylation gene (ermB or ermTR variant A) predicted in vitro constitutive or inducible resistance to clindamycin with a sensitivity of 93% (95% CI 86%-97%), specificity of 90% (95% CI 85%-93%), positive predictive value of 76% (95% CI 67%-84%), and negative predictive value of 97% (95% CI 94%-99%). Conclusion. This rapid and simple assay can predict in vitro susceptibility to clindamycin within two hours of isolation as opposed to 18-24 hours via disk diffusion. The assay might also be used to screen large numbers of batched isolates to establish the prevalence of resistance in a given area.
UR - https://www.scopus.com/pages/publications/34447504309
UR - https://www.scopus.com/inward/citedby.url?scp=34447504309&partnerID=8YFLogxK
U2 - 10.1155/2007/46581
DO - 10.1155/2007/46581
M3 - Article
C2 - 17710240
AN - SCOPUS:34447504309
SN - 1064-7449
VL - 2007
JO - Infectious diseases in obstetrics and gynecology
JF - Infectious diseases in obstetrics and gynecology
M1 - 46581
ER -