Rapid determination of genomic DNA length for new bacteriophages

Philip Serwer, Shirley J. Hayes, Julie A. Thomas, Gary A. Griess, Stephen C. Hardies

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

dsDNA viruses with long genomes (>200 kb) are expected to be a major source of novel genes. To rapidly characterize the genomes of newly isolated dsDNA bacteriophages, we develop here a procedure for the PFGE of intact long DNA genomes from bacteriophage particles in unfractionated, infected cell lysates of either liquid or gelled cultures. The DNA used for PFGE is suitable for sequencing after extraction with phenol. The PFGE is tuned to the range of expected DNA lengths. This procedure bypasses the isolation of bacteriophage particles and is useful for PFGE analysis of DNA from dissected zones of bacteriophage plaques.

Original languageEnglish (US)
Pages (from-to)1896-1902
Number of pages7
JournalELECTROPHORESIS
Volume28
Issue number12
DOIs
StatePublished - Jun 2007

Keywords

  • Agarose gel
  • DNA sequencing
  • Genomic
  • Microbial ecology
  • PFGE

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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