Rapid detection of contaminated intravenous fluids using the Limulus in vitro endotoxin assay

J. H. Jorgensen, R. F. Smith

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Intravenous fluids and administration sets may become contaminated with gram negative bacteria during use and result in a life threatening situation to the patient. The Limulus in vitro assay for endotoxin was used in two patients whose parenteral fluids had become contaminated with Pseudomonas aeruginosa. This test allowed rapid detection of the contaminated intravenous fluids and demonstrated a concomitant endotoxemia in both patients. The same strains of pesudomonas were subsequently cultured from each patient's blood, intravenous catheter tip, and parenteral fluid and administration set. A different serotype of pseudomonas was unique to each patient, indicating two separate and unrelated cases of accidental contamination of the administration sets. Endotoxin like activity was also demonstrated from several brands of commercial human serum albumin, which may contribute low level activity detectable by the Limulus assay.

Original languageEnglish (US)
Pages (from-to)521-524
Number of pages4
JournalJournal of Applied Microbiology
Volume26
Issue number4
StatePublished - 1973
Externally publishedYes

Fingerprint

Limulus
Horseshoe Crabs
endotoxins
Endotoxins
assays
endotoxemia
Endotoxemia
Pseudomonas
Gram-Negative Bacteria
Pseudomonas aeruginosa
Gram-negative bacteria
catheters
Serum Albumin
Intravenous Administration
serotypes
Catheters
fluids
In Vitro Techniques
blood
testing

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Rapid detection of contaminated intravenous fluids using the Limulus in vitro endotoxin assay. / Jorgensen, J. H.; Smith, R. F.

In: Journal of Applied Microbiology, Vol. 26, No. 4, 1973, p. 521-524.

Research output: Contribution to journalArticle

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