Abstract
A pilot study was conducted to determine the feasibility of using the Limulus endotoxin assay to detect endotoxins in potable waters and from reclaimed advanced waste treatment (AWT) plant effluents. Water samples were tested using both Limulus lysates prepared in the laboratory and a commercial product, Difco Pyrotest. The Limulus assay procedure was easily adapted to the testing of water samples for endotoxin. Measured endotoxin concentrations varied from 0.78 ng/ml to 1,250 ng/ml. Levels of endotoxin were not predictable based on whether the water was drinking water or AWT water, i.e., some AWT water samples had less endotoxin activity than some samples of drinking water, and some AWT waters had greater endotoxin activity than drinking water. Only three of the water samples tested were free of any detectable endotoxin. Breakpoint chlorination procedures seemed to reduce measurable endotoxin content, whereas passage through activated carbon columns was associated with greater final endotoxin concentrations in test waters.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 347-351 |
| Number of pages | 5 |
| Journal | Applied and Environmental Microbiology |
| Volume | 32 |
| Issue number | 3 |
| State | Published - 1976 |
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ASJC Scopus subject areas
- Biotechnology
- Environmental Science(all)
- Microbiology
Cite this
Rapid detection of bacterial endotoxins in drinking water and renovated wastewater. / Jorgensen, J. H.; Lee, J. C.; Pahren, H. R.
In: Applied and Environmental Microbiology, Vol. 32, No. 3, 1976, p. 347-351.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid detection of bacterial endotoxins in drinking water and renovated wastewater
AU - Jorgensen, J. H.
AU - Lee, J. C.
AU - Pahren, H. R.
PY - 1976
Y1 - 1976
N2 - A pilot study was conducted to determine the feasibility of using the Limulus endotoxin assay to detect endotoxins in potable waters and from reclaimed advanced waste treatment (AWT) plant effluents. Water samples were tested using both Limulus lysates prepared in the laboratory and a commercial product, Difco Pyrotest. The Limulus assay procedure was easily adapted to the testing of water samples for endotoxin. Measured endotoxin concentrations varied from 0.78 ng/ml to 1,250 ng/ml. Levels of endotoxin were not predictable based on whether the water was drinking water or AWT water, i.e., some AWT water samples had less endotoxin activity than some samples of drinking water, and some AWT waters had greater endotoxin activity than drinking water. Only three of the water samples tested were free of any detectable endotoxin. Breakpoint chlorination procedures seemed to reduce measurable endotoxin content, whereas passage through activated carbon columns was associated with greater final endotoxin concentrations in test waters.
AB - A pilot study was conducted to determine the feasibility of using the Limulus endotoxin assay to detect endotoxins in potable waters and from reclaimed advanced waste treatment (AWT) plant effluents. Water samples were tested using both Limulus lysates prepared in the laboratory and a commercial product, Difco Pyrotest. The Limulus assay procedure was easily adapted to the testing of water samples for endotoxin. Measured endotoxin concentrations varied from 0.78 ng/ml to 1,250 ng/ml. Levels of endotoxin were not predictable based on whether the water was drinking water or AWT water, i.e., some AWT water samples had less endotoxin activity than some samples of drinking water, and some AWT waters had greater endotoxin activity than drinking water. Only three of the water samples tested were free of any detectable endotoxin. Breakpoint chlorination procedures seemed to reduce measurable endotoxin content, whereas passage through activated carbon columns was associated with greater final endotoxin concentrations in test waters.
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UR - http://www.scopus.com/inward/citedby.url?scp=0017095209&partnerID=8YFLogxK
M3 - Article
C2 - 791115
AN - SCOPUS:0017095209
VL - 32
SP - 347
EP - 351
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
SN - 0099-2240
IS - 3
ER -