Rapid detection and characterization of multimolecular cellular constituents by two-dimensional agarose gel electrophoresis

Philip Serwer; Richard A. Easom; Shirley J. Hayes; Merle S. Olson

doi:10.1016/0968-0004(89)90076-5

Rapid detection and characterization of multimolecular cellular constituents by two-dimensional agarose gel electrophoresis

Philip Serwer, Richard A. Easom, Shirley J. Hayes, Merle S. Olson

Biochemistry & Structural Biology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Agarose gel electrophoresis (best known for fractionating nucleic acids by length and conformation) may also be used for fractionating multimolecular complexes of either protein or protein together with other macromolecules. To improve analyses of such complexes, a procedure of two-dimensional agarose gel electrophoresis (2D-AGE) with the following capabilities has been developed: (1) the radius (R) of those spherical particles that form bands can be measured with an accuracy that can be as high as ±1%; (2) the solid-support-free electrophoretic mobility of band-forming particles can be measured; (3) the distribution of R in mixtures heterogeneous in R can be measured; and (4) complex mixtures can be analysed with separations that can be tuned to the R of particles of interest.

Original language	English (US)
Pages (from-to)	4-7
Number of pages	4
Journal	Trends in Biochemical Sciences
Volume	14
Issue number	1
DOIs	https://doi.org/10.1016/0968-0004(89)90076-5
State	Published - Jan 1989

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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title = "Rapid detection and characterization of multimolecular cellular constituents by two-dimensional agarose gel electrophoresis",

abstract = "Agarose gel electrophoresis (best known for fractionating nucleic acids by length and conformation) may also be used for fractionating multimolecular complexes of either protein or protein together with other macromolecules. To improve analyses of such complexes, a procedure of two-dimensional agarose gel electrophoresis (2D-AGE) with the following capabilities has been developed: (1) the radius (R) of those spherical particles that form bands can be measured with an accuracy that can be as high as ±1%; (2) the solid-support-free electrophoretic mobility of band-forming particles can be measured; (3) the distribution of R in mixtures heterogeneous in R can be measured; and (4) complex mixtures can be analysed with separations that can be tuned to the R of particles of interest.",

author = "Philip Serwer and Easom, {Richard A.} and Hayes, {Shirley J.} and Olson, {Merle S.}",

note = "Funding Information: The authors thank Dr Richard F. Luduefia for a generous gift of microtubules, Elena T. Moreno for assistance with experiments, Dr T.J. Morris for a generous gift o:~ the plant viruses used here, and Linda C. Winchester for typing this manuscript. Support was received from the National Institutes of Health (Grants GM-24365 to PS and HL-24654 to MS()) and the",

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doi = "10.1016/0968-0004(89)90076-5",

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T1 - Rapid detection and characterization of multimolecular cellular constituents by two-dimensional agarose gel electrophoresis

AU - Serwer, Philip

AU - Easom, Richard A.

AU - Hayes, Shirley J.

AU - Olson, Merle S.

N1 - Funding Information: The authors thank Dr Richard F. Luduefia for a generous gift of microtubules, Elena T. Moreno for assistance with experiments, Dr T.J. Morris for a generous gift o:~ the plant viruses used here, and Linda C. Winchester for typing this manuscript. Support was received from the National Institutes of Health (Grants GM-24365 to PS and HL-24654 to MS()) and the

PY - 1989/1

Y1 - 1989/1

N2 - Agarose gel electrophoresis (best known for fractionating nucleic acids by length and conformation) may also be used for fractionating multimolecular complexes of either protein or protein together with other macromolecules. To improve analyses of such complexes, a procedure of two-dimensional agarose gel electrophoresis (2D-AGE) with the following capabilities has been developed: (1) the radius (R) of those spherical particles that form bands can be measured with an accuracy that can be as high as ±1%; (2) the solid-support-free electrophoretic mobility of band-forming particles can be measured; (3) the distribution of R in mixtures heterogeneous in R can be measured; and (4) complex mixtures can be analysed with separations that can be tuned to the R of particles of interest.

AB - Agarose gel electrophoresis (best known for fractionating nucleic acids by length and conformation) may also be used for fractionating multimolecular complexes of either protein or protein together with other macromolecules. To improve analyses of such complexes, a procedure of two-dimensional agarose gel electrophoresis (2D-AGE) with the following capabilities has been developed: (1) the radius (R) of those spherical particles that form bands can be measured with an accuracy that can be as high as ±1%; (2) the solid-support-free electrophoretic mobility of band-forming particles can be measured; (3) the distribution of R in mixtures heterogeneous in R can be measured; and (4) complex mixtures can be analysed with separations that can be tuned to the R of particles of interest.

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Rapid detection and characterization of multimolecular cellular constituents by two-dimensional agarose gel electrophoresis

Abstract

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