Abstract
A simple, rapid bioassay for the measurement of chloramphenicol in serum or cerebrospinal fluid was developed using a multiply antibiotic-resistant strain of Escherichia coli. The agar diffusion system involved the addition of patients' specimens and three standard concentrations of chloramphenicol to 7.5 mm diameter wells cut in agar seeded with the test organism. Assays of chloramphenicol using this system could be read routinely in three to four hours and allowed determinations of levels of 5-60 μg/ml. Chloramphenicol could be measured accurately in the presence of a variety of beta-lactam, aminoglycoside, sulfonamide, and tetracycline antimicrobial agents, but not cefoxitin or trimethoprim-sulfamethoxazole. Repetitive assays for sera containing known concentrations of chloramphenicol indicated a coefficient of variation of 8%. Seeded assay plates could be stored at 2-8°C for up to five days prior to use.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 472-475 |
| Number of pages | 4 |
| Journal | American Journal of Clinical Pathology |
| Volume | 76 |
| Issue number | 4 |
| State | Published - 1981 |
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ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Rapid bioassay for chloramphenicol in the presence of other antibiotics. / Jorgensen, J. H.; Alexander, G. A.
In: American Journal of Clinical Pathology, Vol. 76, No. 4, 1981, p. 472-475.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid bioassay for chloramphenicol in the presence of other antibiotics
AU - Jorgensen, J. H.
AU - Alexander, G. A.
PY - 1981
Y1 - 1981
N2 - A simple, rapid bioassay for the measurement of chloramphenicol in serum or cerebrospinal fluid was developed using a multiply antibiotic-resistant strain of Escherichia coli. The agar diffusion system involved the addition of patients' specimens and three standard concentrations of chloramphenicol to 7.5 mm diameter wells cut in agar seeded with the test organism. Assays of chloramphenicol using this system could be read routinely in three to four hours and allowed determinations of levels of 5-60 μg/ml. Chloramphenicol could be measured accurately in the presence of a variety of beta-lactam, aminoglycoside, sulfonamide, and tetracycline antimicrobial agents, but not cefoxitin or trimethoprim-sulfamethoxazole. Repetitive assays for sera containing known concentrations of chloramphenicol indicated a coefficient of variation of 8%. Seeded assay plates could be stored at 2-8°C for up to five days prior to use.
AB - A simple, rapid bioassay for the measurement of chloramphenicol in serum or cerebrospinal fluid was developed using a multiply antibiotic-resistant strain of Escherichia coli. The agar diffusion system involved the addition of patients' specimens and three standard concentrations of chloramphenicol to 7.5 mm diameter wells cut in agar seeded with the test organism. Assays of chloramphenicol using this system could be read routinely in three to four hours and allowed determinations of levels of 5-60 μg/ml. Chloramphenicol could be measured accurately in the presence of a variety of beta-lactam, aminoglycoside, sulfonamide, and tetracycline antimicrobial agents, but not cefoxitin or trimethoprim-sulfamethoxazole. Repetitive assays for sera containing known concentrations of chloramphenicol indicated a coefficient of variation of 8%. Seeded assay plates could be stored at 2-8°C for up to five days prior to use.
UR - http://www.scopus.com/inward/record.url?scp=0019481687&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019481687&partnerID=8YFLogxK
M3 - Article
C2 - 7027784
AN - SCOPUS:0019481687
VL - 76
SP - 472
EP - 475
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
SN - 0002-9173
IS - 4
ER -