Rapid bioassay for chloramphenicol in the presence of other antibiotics

J. H. Jorgensen, G. A. Alexander

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A simple, rapid bioassay for the measurement of chloramphenicol in serum or cerebrospinal fluid was developed using a multiply antibiotic-resistant strain of Escherichia coli. The agar diffusion system involved the addition of patients' specimens and three standard concentrations of chloramphenicol to 7.5 mm diameter wells cut in agar seeded with the test organism. Assays of chloramphenicol using this system could be read routinely in three to four hours and allowed determinations of levels of 5-60 μg/ml. Chloramphenicol could be measured accurately in the presence of a variety of beta-lactam, aminoglycoside, sulfonamide, and tetracycline antimicrobial agents, but not cefoxitin or trimethoprim-sulfamethoxazole. Repetitive assays for sera containing known concentrations of chloramphenicol indicated a coefficient of variation of 8%. Seeded assay plates could be stored at 2-8°C for up to five days prior to use.

Original languageEnglish (US)
Pages (from-to)472-475
Number of pages4
JournalAmerican Journal of Clinical Pathology
Volume76
Issue number4
StatePublished - 1981

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Chloramphenicol
Biological Assay
Anti-Bacterial Agents
Agar
Cefoxitin
Sulfonamides
Sulfamethoxazole Drug Combination Trimethoprim
beta-Lactams
Aminoglycosides
Anti-Infective Agents
Tetracycline
Serum
Cerebrospinal Fluid
Escherichia coli

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Rapid bioassay for chloramphenicol in the presence of other antibiotics. / Jorgensen, J. H.; Alexander, G. A.

In: American Journal of Clinical Pathology, Vol. 76, No. 4, 1981, p. 472-475.

Research output: Contribution to journalArticle

Jorgensen, J. H. ; Alexander, G. A. / Rapid bioassay for chloramphenicol in the presence of other antibiotics. In: American Journal of Clinical Pathology. 1981 ; Vol. 76, No. 4. pp. 472-475.
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