Rapid analysis of DNA methylation using new restriction enzyme sites created by bisulfite modification

Ramin Sadri, Peter J. Hornsby

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

Bisulfite converts non-methylated cytosine in DNA to uracil leaving 5-methylcytosine unaltered. Here, predicted changes in restriction enzyme sites following reaction of genomic DNA with bisulfite and amplification of the product by the polymerase chain reaction (PCR) were used to assess the methylation of CpG sites. This procedure differs from conventional DNA methylation analysis by methylation-sensitive restriction enzymes because it does not rely on an absence of cleavage to detect methylated sites, the two strands of DNA produce different restriction enzyme sites and may be differentially analyzed, and closely related sequences may be separately analyzed by using specific PCR primers.

Original languageEnglish (US)
Pages (from-to)5058-5059
Number of pages2
JournalNucleic acids research
Volume24
Issue number24
DOIs
StatePublished - Dec 15 1996
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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