Rapid 3D NMR using the filter diagonalization method: Application to oligosaccharides derivatized with 13C-labeled acetyl groups

Geoffrey S. Armstrong, Kristin E. Cano, Vladimir A. Mandelshtam, A. J. Shaka, Brad Bendiak

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Rapid 3D NMR spectroscopy of oligosaccharides having isotopically labeled acetyl "isotags" was made possible with high resolution in the indirect dimensions using the filter diagonalization method (FDM). A pulse sequence was designed for the optimal correlation of acetyl methyl protons, methyl carbons, and carbonyl carbons. The multi-dimensional nature of the FDM, coupled with the advantages of constant-time evolution periods, resulted in marked improvements over Fourier transform (FT) and mirror-image linear prediction (MI-LP) processing methods. The three methods were directly compared using identical data sets. A highly resolved 3D spectrum was achieved with the FDM using a very short experimental time (28min).

Original languageEnglish (US)
Pages (from-to)156-163
Number of pages8
JournalJournal of Magnetic Resonance
Volume170
Issue number1
DOIs
StatePublished - Sep 2004
Externally publishedYes

Keywords

  • C-13 labeling
  • Constant-time
  • Filter diagonalization method
  • Multi-dimensional NMR
  • O-acetylation
  • Oligosaccharides

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Nuclear and High Energy Physics
  • Biophysics
  • Biochemistry

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