Abstract
Rapid 3D NMR spectroscopy of oligosaccharides having isotopically labeled acetyl "isotags" was made possible with high resolution in the indirect dimensions using the filter diagonalization method (FDM). A pulse sequence was designed for the optimal correlation of acetyl methyl protons, methyl carbons, and carbonyl carbons. The multi-dimensional nature of the FDM, coupled with the advantages of constant-time evolution periods, resulted in marked improvements over Fourier transform (FT) and mirror-image linear prediction (MI-LP) processing methods. The three methods were directly compared using identical data sets. A highly resolved 3D spectrum was achieved with the FDM using a very short experimental time (28min).
Original language | English (US) |
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Pages (from-to) | 156-163 |
Number of pages | 8 |
Journal | Journal of Magnetic Resonance |
Volume | 170 |
Issue number | 1 |
DOIs | |
State | Published - Sep 2004 |
Externally published | Yes |
Keywords
- C-13 labeling
- Constant-time
- Filter diagonalization method
- Multi-dimensional NMR
- O-acetylation
- Oligosaccharides
ASJC Scopus subject areas
- Condensed Matter Physics
- Nuclear and High Energy Physics
- Biophysics
- Biochemistry