TY - JOUR
T1 - RAP80/UIMC1 as cancer-associated antigen
T2 - Alternative splice variants and their immunogenicity
AU - Shebzukhov, Yuriy V.
AU - Koroleva, Ekaterina P.
AU - Khlgatian, Svetlana V.
AU - Belousov, Pavel V.
AU - Sazykin, Alexey Y.
AU - Kadachigova, Tatiana S.
AU - Pomerantseva, Ekaterina A.
AU - Lagarkova, Maria A.
AU - Nedospasov, Sergei A.
AU - Kuprash, Dmitry V.
N1 - Funding Information:
This study was performed in the frame of Cancer Antigen Discovery Collaborative of the Cancer Research Institute and the Ludwig Institute for Cancer Research. It was additionally supported by the MCB grants from the Russian Academy of Sciences and by Russian Foundation for Basic Research (Grant 05-04-49075-a).
PY - 2007/10/8
Y1 - 2007/10/8
N2 - We have identified RAP80/UIMC1, the protein highly expressed in testis, as a new cancer-associated antigen. Sera from 5% to 10% of patients with different types of cancer contain specific antibodies to RAP80/UIMC1. In order to investigate the possible reasons for RAP80/UIMC1 immunogenicity, we characterized its numerous splice isoforms and mapped immunogenic regions of the protein. The majority of RAP80/UIMC1 transcripts was detected both in normal tissues and in colon tumors. There are several RAP80/UIMC1 isoforms that are predominantly expressed in testis, however we did not observe elevated expression of these transcripts in tumors from seropositive patients. We mapped the major immunogenic region of RAP80/UIMC1 to the central part of the protein encoded by exon 9 which is present in a number of ubiquitous splice forms. Thus, based on our data, autoreactivity to RAP80/UIMC1 is related to reasons other than overexpression or tumor-specific splicing.
AB - We have identified RAP80/UIMC1, the protein highly expressed in testis, as a new cancer-associated antigen. Sera from 5% to 10% of patients with different types of cancer contain specific antibodies to RAP80/UIMC1. In order to investigate the possible reasons for RAP80/UIMC1 immunogenicity, we characterized its numerous splice isoforms and mapped immunogenic regions of the protein. The majority of RAP80/UIMC1 transcripts was detected both in normal tissues and in colon tumors. There are several RAP80/UIMC1 isoforms that are predominantly expressed in testis, however we did not observe elevated expression of these transcripts in tumors from seropositive patients. We mapped the major immunogenic region of RAP80/UIMC1 to the central part of the protein encoded by exon 9 which is present in a number of ubiquitous splice forms. Thus, based on our data, autoreactivity to RAP80/UIMC1 is related to reasons other than overexpression or tumor-specific splicing.
KW - Colon cancer
KW - SEREX
KW - Transcription
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U2 - 10.1016/j.canlet.2007.04.013
DO - 10.1016/j.canlet.2007.04.013
M3 - Article
C2 - 17562356
AN - SCOPUS:34548029998
SN - 0304-3835
VL - 255
SP - 255
EP - 262
JO - Cancer Letters
JF - Cancer Letters
IS - 2
ER -