@article{2890bc173a3a4b1bafd04a005d7fac2d,
title = "RalA controls glucose homeostasis by regulating glucose uptake in brown fat",
abstract = "Insulin increases glucose uptake into adipose tissue and muscle by increasing trafficking of the glucose transporter Glut4. In cultured adipocytes, the exocytosis of Glut4 relies on activation of the small G protein RalA by insulin, via inhibition of its GTPase activating complex RalGAP. Here, we evaluate the role of RalA in glucose uptake in vivo with specific chemical inhibitors and by generation of mice with adipocyte-specific knockout of RalGAPB. RalA was profoundly activated in brown adipose tissue after feeding, and its inhibition prevented Glut4 exocytosis. RalGAPB knockout mice with diet-induced obesity were protected from the development of metabolic disease due to increased glucose uptake into brown fat. Thus, RalA plays a crucial role in glucose transport in adipose tissue in vivo.",
keywords = "Glut4, GTPase, insulin, Ral inhibitors, RalGAP",
author = "Yuliya Skorobogatko and Morgan Dragan and Claudia Cordon and Reilly, {Shannon M.} and Hung, {Chao Wei} and Wenmin Xia and Peng Zhao and Martina Wallace and Lackey, {Denise E.} and Chen, {Xiao Wei} and Olivia Osborn and Bogner-Strauss, {Juliane G.} and Dan Theodorescu and Metallo, {Christian M.} and Olefsky, {Jerrold M.} and Saltiel, {Alan R.}",
note = "Funding Information: ACKNOWLEDGMENTS. We thank the Metabolomics Core at the University of Michigan for performing NMR measurements of body composition; the Histology Core at the University of Michigan School of Dentistry; the Histology Core at Moores Cancer Center at the University of California, San Diego; and the Biomolecular and Proteomics Mass Spectrometry Facility at the University of California, San Diego. This work was supported by NIH Grants DK076906, DK061618, and DK060591 (to A.R.S.); P30 DK063491, 16POST27740033 (to Y.S.); and ADA 1-18-PDF-094 (to C.-W.H). Funding Information: We thank the Metabolomics Core at the University of Michigan for performing NMR measurements of body composition; the Histology Core at the University of Michigan School of Dentistry; the Histology Core at Moores Cancer Center at the University of California, San Diego; and the Biomolecular and Proteomics Mass Spectrometry Facility at the University of California, San Diego. This work was supported by NIH Grants DK076906, DK061618, and DK060591 (to A.R.S.); P30 DK063491, 16POST27740033 (to Y.S.); and ADA 1-18-PDF-094 (to C.-W.H). Publisher Copyright: {\textcopyright} 2018 National Academy of Sciences. All Rights Reserved.",
year = "2018",
month = jul,
day = "24",
doi = "10.1073/pnas.1801050115",
language = "English (US)",
volume = "115",
pages = "7819--7824",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "30",
}