Radiolabeling of nanoshells with 64Cu and 111In for PET and SPECT imaging in rats

Xie Huan, Jim Wang Zheng, Bao Ande, Goins Beth, T. Phillips William, L. Coleman Chris

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Here we report for the first time the radioactive labeling of gold nanoshells with 64Cu and 111In. A conjugation method was developed to link nanoshells with the radioactive tags. The resulting conjugates showed good labeling efficiency and stability in PBS and serum for in vivo imaging applications. The circulation of 64Cu-nanoshells and 111In-nanoshells in mice were determined by dynamic light scattering and their blood clearance in rats were determined in terms of radioactive counting. By PET/CT and SPECT/CT imaging, we monitored the in vivo distribution of 64Cu-nanoshells and 111In-nanoshells in nude rats with a head and neck squamous cell carcinoma xenograft at various time points after their intravenous injection. PET and SPECT images of the rats showed that both of the labeled nanoshells accumulated in the tumor. PET images with 64Cu showed better resolution and were therefore, more promising for future applications.

Original languageEnglish (US)
Title of host publicationTechnical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
Pages1-4
Number of pages4
StatePublished - 2009
EventNanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 - Houston, TX, United States
Duration: May 3 2009May 7 2009

Publication series

NameTechnical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
Volume2

Other

OtherNanotechnology 2009: Life Sciences, Medicine, Diagnostics, Bio Materials and Composites - 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009
CountryUnited States
CityHouston, TX
Period5/3/095/7/09

Keywords

  • Cancer
  • Nanoshells
  • PET
  • Radiolabeling
  • SPECT

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering
  • Biomaterials

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    Huan, X., Zheng, J. W., Ande, B., Beth, G., William, T. P., & Chris, L. C. (2009). Radiolabeling of nanoshells with 64Cu and 111In for PET and SPECT imaging in rats. In Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009 (pp. 1-4). (Technical Proceedings of the 2009 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2009; Vol. 2).