Radioimmunoassay of 3α-hydroxy-5α-pregnan-20-one in rat and human plasma

Robert H. Purdy, Perry H. Moore, P. Narasimha Rao, Nobuyoshi Hagino, Tatsumi Yamaguchi, Peter Schmidt, David R. Rubinow, A. Leslie Morrow, Steven M. Paul

Research output: Contribution to journalArticlepeer-review

200 Scopus citations

Abstract

A radioimmunoassay for measuring 3α-hydroxy-5α-pregnan-20-one in plasma has been developed. Polyclonal antibodies were raised in rabbits against 3α-hydroxy-20-oxo-5α-pregnan-11α-yl carboxymethyl ether coupled to bovine serum albumin. 3α-Hydroxy-5α-pregnan-20-one was purified from ether extracts of plasma by high-performance liquid chromatography. These antibodies were then used for the radioimmunoassay of this centrally active progesterone metabolite in rat and human plasma. 3α-Hydroxy-5α-pregnan-20-one was detected in plasma from female rats on the day ofestrus (2.0 to 9.3 ng/ml) and in the plasma of women during the luteal phase of the menstrual cycle at levels ranging from 0.25 to 2.5 ng/ml. The latter was highly correlated with plasma progesterone levels.

Original languageEnglish (US)
Pages (from-to)290-296
Number of pages7
JournalSteroids
Volume55
Issue number7
DOIs
StatePublished - Jul 1990
Externally publishedYes

Keywords

  • allopregnanolone
  • plasma RIA
  • progesterone
  • steroids

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry

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