A rapid, easy radioassay for serum vitamin B12 is described. Saliva, a readily available, rich source of vitamin B12-binding protein, is used. The assay is reproducible, correlates satisfactorily with microbiological assay results, and clearly differentiates between B12-deficient, normal, and high-B12 sera. Poor exclusion by some hemoglobin-coated charcoals of saliva-bound B12, in the absence of serum resulted in falsely low results. This problem can be corrected by acidification and redrying of the charcoal powder prior to coating or circumvented by additional coating of the hemoglobin-coated charcoal with vitamin B12-saturated serum.
|Original language||English (US)|
|Number of pages||9|
|Journal||The Journal of Laboratory and Clinical Medicine|
|State||Published - Dec 1969|
ASJC Scopus subject areas
- Pathology and Forensic Medicine