Radiation-induced changes of structural and functional properties of human hemoglobin - II. Structural and functional characterization of irradiated deoxyhemoglobin

Z. Szweda-Lewandowska; M. Puchała; P. A. Osmulski; J. Rosin

doi:10.1007/BF01209722

Radiation-induced changes of structural and functional properties of human hemoglobin - II. Structural and functional characterization of irradiated deoxyhemoglobin

Z. Szweda-Lewandowska, M. Puchała, P. A. Osmulski, J. Rosin

Research output: Contribution to journal › Article › peer-review

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Abstract

Gel filtration and SDS-PAGE separation of hemoglobin (Hb) irradiated under argon or N₂O show formation of covalent-aggregated Hb molecules. The production of covalent bonds is attributed mainly to the action of hydroxyl radicals, because addition of ethanol, a scavenger of these radicals, suppresses this reaction to a great extent. The oxidized heme iron forming metHb or hemichromes is found in all the separated fractions of irradiated Hb. It is also found that the radiation-modified Hb molecules exhibit a decrease of co-operative binding of oxygen.

Original language	English (US)
Pages (from-to)	47-58
Number of pages	12
Journal	Radiation and Environmental Biophysics
Volume	28
Issue number	1
DOIs	https://doi.org/10.1007/BF01209722
State	Published - Mar 1989
Externally published	Yes

ASJC Scopus subject areas

General Environmental Science
Radiation
Biophysics

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Radiation-induced changes of structural and functional properties of human hemoglobin - II. Structural and functional characterization of irradiated deoxyhemoglobin. / Szweda-Lewandowska, Z.; Puchała, M.; Osmulski, P. A. et al.
In: Radiation and Environmental Biophysics, Vol. 28, No. 1, 03.1989, p. 47-58.

Research output: Contribution to journal › Article › peer-review

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AU - Osmulski, P. A.

AU - Rosin, J.

PY - 1989/3

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Radiation-induced changes of structural and functional properties of human hemoglobin - II. Structural and functional characterization of irradiated deoxyhemoglobin

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