@inbook{ccad6745ad664bf2bde7f830b1e6a779,
title = "RADD: A real-time FRET-based biochemical assay for DNA deaminase studies",
abstract = "In recent years, the connection between APOBEC3 cytosine deaminases and cancer mutagenesis has become ever more apparent. This growing awareness and lack of inhibitory drugs has created a distinct need for biochemical tools that can be used to identify and characterize potential inhibitors of this family of enzymes. In response to this challenge, we have developed a Real-time APOBEC3-mediated DNA Deamination (RADD) assay. The RADD assay provides a rapid, real-time fluorescence readout of APOBEC3 DNA deamination and serves as a crucial addition to the existing APOBEC3 biochemical and cellular toolkit. This method improves upon contemporary DNA deamination assays by offering a more rapid and quantifiable readout as well as providing a platform that is readily adaptable to a high-throughput format for inhibitor discovery. In this chapter we provide a detailed guide for the usage of the RADD assay for the characterization of APOBEC3 enzymes and potential inhibitors.",
keywords = "APOBEC3, Cancer, Cytidine deaminase, Fluorescence resonance energy transfer (FRET), High-throughput screening (HTS), Real-time assay",
author = "Belica, \{Christopher A.\} and Hernandez, \{Patricia C.\} and Carpenter, \{Michael A.\} and Yanjun Chen and Brown, \{William L.\} and Harris, \{Reuben S.\} and Hideki Aihara",
note = "Publisher Copyright: {\textcopyright} 2024",
year = "2024",
month = jan,
doi = "10.1016/bs.mie.2024.08.001",
language = "English (US)",
isbn = "9780443297243",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "311--345",
editor = "Eichman, \{Brandt F.\}",
booktitle = "Chemical and Genomic Methods in Nucleic Acid Biology",
address = "United States",
}