@article{0b9515eb1f6f4ce2aefa0d1cc38074d0,
title = "Rad54 protein is targeted to pairing loci by the Rad51 nucleoprotein filament",
abstract = "Rad51 and Rad54 proteins are important for the repair of double-stranded DNA (dsDNA) breaks by homologous recombination in eukaryotes. Rad51 assembles on single-stranded DNA (ssDNA) to form a helical nucleoprotein filament that performs homologous pairing with dsDNA; Rad54 stimulates this pairing substantially. Here, we demonstrate that Rad54 acts in concert with the mature Rad51-ssDNA filament. Enhancement of DNA pairing by Rad54 is greatest at an equimolar ratio relative to Rad51 within the filament. Reciprocally, the Rad51-ssDNA filament enhances both the dsDNA-dependent ATPase and the dsDNA unwinding activities of Rad54. We conclude that Rad54 participates in the DNA homology search as a component of the Rad51-nucleoprotein filament and that the filament delivers Rad54 to the dsDNA pairing locus, thereby linking the unwinding of potential target DNA with the homology search process.",
author = "Mazin, {Alexander V.} and Bornarth, {Carole J.} and Solinger, {Jachen A.} and Heyer, {Wolf Dietrich} and Kowalczykowski, {Stephen C.}",
note = "Funding Information: We are grateful to Piero Bianco, Jason Churchill, Frank Harmon, Cynthia Haseltine, Noriko Kantake, Konstantin Kiianitsa, Julie Kleiman, James New, Erica Seitz, and Tomohiko Sugiyama for their comments on the manuscript. We are especially grateful to Patrick Sung (The University of Texas Health Science Center at San Antonio) for providing human Rad51 protein. We thank Shunichi Takeda for communication of data prior to publication. A. M. is grateful to the Institute of Cytology and Genetics, Russian Academy of Science, Novosibirsk, for granting him a leave of absence. This work was supported by the following grants: National Institutes of Health (NIH) (AI-18987) and Human Frontiers Science Program (RG63) to S. C. K.; NIH Postdoctoral Fellowship (GM-20057) to C. J. B.; and Swiss National Science Foundation (31-4556495), NIH (GM-58015), and Human Frontier Science Program (RG63) to W.-D. H.",
year = "2000",
doi = "10.1016/S1097-2765(00)00057-5",
language = "English (US)",
volume = "6",
pages = "583--592",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "3",
}