Rad54 and Rdh54 prevent Srs2-mediated disruption of Rad51 presynaptic filaments

Aviv Meir, J. Brooks Crickard, Youngho Kwon, Patrick Sung, Eric C. Greene

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Srs2 is a superfamily 1 (SF1) helicase that participates in several pathways necessary for the repair of damaged DNA. Srs2 regulates formation of early homologous recombination (HR) intermediates by actively removing the recombinase Rad51 from single-stranded DNA (ssDNA). It is not known whether and how Srs2 itself is down-regulated to allow for timely HR progression. Rad54 and Rdh54 are two closely related superfamily 2 (SF2) motor proteins that promote the formation of Rad51-dependent recombination intermediates. Rad54 and Rdh54 bind tightly to Rad51-ssDNA and act downstream of Srs2, suggesting that they may affect the ability of Srs2 to dismantle Rad51 filaments. Here, we used DNA curtains to determine whether Rad54 and Rdh54 alter the ability of Srs2 to disrupt Rad51 filaments. We show that Rad54 and Rdh54 act synergistically to greatly restrict the antirecombinase activity of Srs2. Our findings suggest that Srs2 may be accorded only a limited time window to act and that Rad54 and Rdh54 fulfill a role of prorecombinogenic licensing factors.

Original languageEnglish (US)
Article numbere2113871119
JournalProceedings of the National Academy of Sciences of the United States of America
Volume119
Issue number4
DOIs
StatePublished - Jan 25 2022

Keywords

  • Homologous recombination
  • Rad51
  • Rad54
  • Rdh54
  • Srs2

ASJC Scopus subject areas

  • General

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