Rad51-independent break-induced replication to repair a broken chromosome depends on a distant enhancer site

Anna Malkova, Laurence Signon, Christopher B. Schaefer, Maria L. Naylor, James F. Theis, Carol S. Newlon, James E. Haber

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

Without the RAD51 strand exchange protein, Saccharomyces cerevisiae cannot repair a double-strand break (DSB) by gene conversion. However, cells can repair DSBs by recombination-dependent, break-induced replication (BIR). RAD51-independent BIR is initiated more than 13 kb from the DSB. Repair depends on a 200-bp sequence adjacent to ARS310, located -34 kb centromere-proximal to the DSB, but does not depend on the origin activity of ARS310. We conclude that the ability of a recombination-induced replication fork to copy >130 kb to the end of the chromosome depends on a special site that enhances assembly of a processive repair replication fork.

Original languageEnglish (US)
Pages (from-to)1055-1060
Number of pages6
JournalGenes and Development
Volume15
Issue number9
DOIs
StatePublished - May 1 2001
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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