Rabbit blastocysts accumulate platelet‐activating factor (PAF) and lyso‐PAF in vitro

M. A. Jones, G. B. Kudolo, M. J.K. Harper

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Platelet‐activating factor (1‐O‐alkyl‐2‐acetyl‐sn‐glycero‐3‐phosphorylcholine; PAF) is a very potent phospholipid, which has been demonstrated to stimulate smooth muscle and change vascular permeability. PAF has been detected in the rabbit preimplantation uterine endometrium and has been demonstrated to bind specifically to rabbit uterine membranes. To evaluate the possible role of PAF in maternal‐embryonic chemical communication, we report here that rabbit blastocysts can accumulate [3H]PAF from their environment. Blastocysts were able to accumulate [3H]PAF as time‐, buffer‐, age‐, and concentration‐dependent functions. The accumulation was inhibited by some PAF receptor antagonists, such as U66985, as well as by unlabeled PAF and lyso‐PAF, indicating that the accumulation process may be receptor mediated. The data support the current model of PAF as a paracrine factor in preimplantation stages of reproduction.

Original languageEnglish (US)
Pages (from-to)243-250
Number of pages8
JournalMolecular Reproduction and Development
Volume32
Issue number3
DOIs
StatePublished - Jul 1992

Keywords

  • PAF
  • Phospholipid
  • Receptors

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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