Rabbit blastocysts accumulate platelet‐activating factor (PAF) and lyso‐PAF in vitro

M. A. Jones; G. B. Kudolo; M. J.K. Harper

doi:10.1002/mrd.1080320309

Rabbit blastocysts accumulate platelet‐activating factor (PAF) and lyso‐PAF in vitro

M. A. Jones, G. B. Kudolo, M. J.K. Harper

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Platelet‐activating factor (1‐O‐alkyl‐2‐acetyl‐sn‐glycero‐3‐phosphorylcholine; PAF) is a very potent phospholipid, which has been demonstrated to stimulate smooth muscle and change vascular permeability. PAF has been detected in the rabbit preimplantation uterine endometrium and has been demonstrated to bind specifically to rabbit uterine membranes. To evaluate the possible role of PAF in maternal‐embryonic chemical communication, we report here that rabbit blastocysts can accumulate [³H]PAF from their environment. Blastocysts were able to accumulate [³H]PAF as time‐, buffer‐, age‐, and concentration‐dependent functions. The accumulation was inhibited by some PAF receptor antagonists, such as U66985, as well as by unlabeled PAF and lyso‐PAF, indicating that the accumulation process may be receptor mediated. The data support the current model of PAF as a paracrine factor in preimplantation stages of reproduction.

Original language	English (US)
Pages (from-to)	243-250
Number of pages	8
Journal	Molecular Reproduction and Development
Volume	32
Issue number	3
DOIs	https://doi.org/10.1002/mrd.1080320309
State	Published - Jul 1992

Keywords

PAF
Phospholipid
Receptors

ASJC Scopus subject areas

Genetics
Cell Biology
Developmental Biology

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10.1002/mrd.1080320309

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title = "Rabbit blastocysts accumulate platelet‐activating factor (PAF) and lyso‐PAF in vitro",

abstract = "Platelet‐activating factor (1‐O‐alkyl‐2‐acetyl‐sn‐glycero‐3‐phosphorylcholine; PAF) is a very potent phospholipid, which has been demonstrated to stimulate smooth muscle and change vascular permeability. PAF has been detected in the rabbit preimplantation uterine endometrium and has been demonstrated to bind specifically to rabbit uterine membranes. To evaluate the possible role of PAF in maternal‐embryonic chemical communication, we report here that rabbit blastocysts can accumulate [3H]PAF from their environment. Blastocysts were able to accumulate [3H]PAF as time‐, buffer‐, age‐, and concentration‐dependent functions. The accumulation was inhibited by some PAF receptor antagonists, such as U66985, as well as by unlabeled PAF and lyso‐PAF, indicating that the accumulation process may be receptor mediated. The data support the current model of PAF as a paracrine factor in preimplantation stages of reproduction.",

keywords = "PAF, Phospholipid, Receptors",

author = "Jones, {M. A.} and Kudolo, {G. B.} and Harper, {M. J.K.}",

year = "1992",

month = jul,

doi = "10.1002/mrd.1080320309",

language = "English (US)",

volume = "32",

pages = "243--250",

journal = "Molecular Reproduction and Development",

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TY - JOUR

T1 - Rabbit blastocysts accumulate platelet‐activating factor (PAF) and lyso‐PAF in vitro

AU - Jones, M. A.

AU - Kudolo, G. B.

AU - Harper, M. J.K.

PY - 1992/7

Y1 - 1992/7

N2 - Platelet‐activating factor (1‐O‐alkyl‐2‐acetyl‐sn‐glycero‐3‐phosphorylcholine; PAF) is a very potent phospholipid, which has been demonstrated to stimulate smooth muscle and change vascular permeability. PAF has been detected in the rabbit preimplantation uterine endometrium and has been demonstrated to bind specifically to rabbit uterine membranes. To evaluate the possible role of PAF in maternal‐embryonic chemical communication, we report here that rabbit blastocysts can accumulate [3H]PAF from their environment. Blastocysts were able to accumulate [3H]PAF as time‐, buffer‐, age‐, and concentration‐dependent functions. The accumulation was inhibited by some PAF receptor antagonists, such as U66985, as well as by unlabeled PAF and lyso‐PAF, indicating that the accumulation process may be receptor mediated. The data support the current model of PAF as a paracrine factor in preimplantation stages of reproduction.

AB - Platelet‐activating factor (1‐O‐alkyl‐2‐acetyl‐sn‐glycero‐3‐phosphorylcholine; PAF) is a very potent phospholipid, which has been demonstrated to stimulate smooth muscle and change vascular permeability. PAF has been detected in the rabbit preimplantation uterine endometrium and has been demonstrated to bind specifically to rabbit uterine membranes. To evaluate the possible role of PAF in maternal‐embryonic chemical communication, we report here that rabbit blastocysts can accumulate [3H]PAF from their environment. Blastocysts were able to accumulate [3H]PAF as time‐, buffer‐, age‐, and concentration‐dependent functions. The accumulation was inhibited by some PAF receptor antagonists, such as U66985, as well as by unlabeled PAF and lyso‐PAF, indicating that the accumulation process may be receptor mediated. The data support the current model of PAF as a paracrine factor in preimplantation stages of reproduction.

KW - PAF

KW - Phospholipid

KW - Receptors

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DO - 10.1002/mrd.1080320309

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JO - Molecular Reproduction and Development

JF - Molecular Reproduction and Development

IS - 3

ER -

Rabbit blastocysts accumulate platelet‐activating factor (PAF) and lyso‐PAF in vitro

Abstract

Keywords

ASJC Scopus subject areas

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