Quinine inhibits multiple Na+ and K+ transport mechanisms in Ehrlich ascites tumor cells

Thomas C. Smith, Charles Levinson

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The interaction of quinine with K+ and Na+ transport mechanisms has been investigated in Ehrlich ascites tumor cells. Quinine affects both Ca2+-dependent K+ channel and total K+ influx. Activation of Ca+-dependent K+ channels by propranolol is abolished by quinine (1 mM). In addition, quinine inhibits the ouabain-sensitive component of K+ influx with an apparent Ki of 0.32 ± 0.02 mM and the furosemide-sensitive component with a Ki of 0.24 ± 0.01 mM. Furthermore, a significant fraction (52%) of Na+ influx is inhibited by quinine. The same component is sensitive to amiloride, suggesting that it represents Na+/H+ antiport. Concomitant with the inhibition of K+ and Na+ transport, quinine stimulates ATP hydrolysis by 57%. The results suggest that quinine exerts broad, nonspecific effects on cellular mechanisms which serve to regulate cation transport in Ehrlich cells.

Original languageEnglish (US)
Pages (from-to)169-175
Number of pages7
JournalBBA - Biomembranes
Volume978
Issue number1
DOIs
StatePublished - Jan 16 1989
Externally publishedYes

Keywords

  • (Ehrlich cell)
  • Calcium ion dependent transport
  • Cation transport
  • Potassium ion transport
  • Quinine
  • Sodium ion transport

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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