Quaternized chitosans bind onto preexisting biofilms and eradicate pre-attached microorganisms

Fuguang Jiang, Ying Deng, Chih-ko Yeh, Yuyu Sun

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Quaternized chitosans, N,N,N-trimethylchitosan (TMC), with different degrees of quaternization were synthesized by reacting methyl iodide with chitosan. The reaction was confirmed by FT-IR and 1H-NMR characterization. Antimicrobial assay showed that the prepared TMC had potent biocidal effects against planktonic Gram-positive bacteria Staphylococcus epidermidis, Gram-negative bacteria Escherichia coli, and yeast Candida albicans. Bacterial and fungal biofilms were formed on poly(methyl methacrylate) (PMMA) films and then treated with TMC aqueous solution. Zeta potential measurement suggested that TMC bonded onto the preexisting biofilms. Biofilm-binding kinetics was evaluated in UV studies using phenyl group-labeled TMC as a model compound, which revealed that quaternized chitosans bonded onto the preexisting biofilms rapidly. Colony-forming unit (CFU) determination and SEM, confocal laser scanning microscopy (CLSM) and fluorescence microscopy studies demonstrated that the bonded TMC had powerful biocidal activities to eradicate the pre-attached bacterial and fungal cells in the preexisting biofilms. The biocompatibility of the TMC samples with rat skin fibroblast cells was evaluated in the MTT assay.

Original languageEnglish (US)
Pages (from-to)8518-8527
Number of pages10
JournalJournal of Materials Chemistry B
Volume2
Issue number48
DOIs
StatePublished - Dec 28 2014

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Chitosan
Biofilms
Microorganisms
Assays
Bacteria
Staphylococcus epidermidis
Candida
Fluorescence microscopy
Gram-Positive Bacteria
Polymethyl Methacrylate
Zeta potential
Fibroblasts
Gram-Negative Bacteria
Candida albicans
Polymethyl methacrylates
Biocompatibility
Fluorescence Microscopy
Confocal Microscopy
Yeast
Escherichia coli

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine(all)
  • Chemistry(all)
  • Materials Science(all)

Cite this

Quaternized chitosans bind onto preexisting biofilms and eradicate pre-attached microorganisms. / Jiang, Fuguang; Deng, Ying; Yeh, Chih-ko; Sun, Yuyu.

In: Journal of Materials Chemistry B, Vol. 2, No. 48, 28.12.2014, p. 8518-8527.

Research output: Contribution to journalArticle

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