Quantitative removal of hepatitis B viral antigens from serum by a monoclonal IgM coupled to a biocompatible solid-phase support

Robert A Marciniak, J. R. Wands, R. R. Bruns, P. S. Malchesky, Y. Nose, E. Haber

Research output: Contribution to journalArticle

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Abstract

We covalently linked to regenerated cellulose filters a high-affinity monoclonal IgM produced against epitopes that reside on hepatitis B viral surface antigen (HBsAg). Conditions were established whereby as much as 250 μg of anti-HBsAgIgM could be linked to 2-4 mg of regenerated cellulose acetate by using cyanogen bromide and trichloro-s-triazine coupling agents. The immunoreactivity of the monoclonal anti-HBsAg IgM was preserved, and quantitative binding studies with HBsAg suggests that more than one functional binding site on the IgM molecule was operative. The specificity of the monoclonal anti-HBsAg IgM was established by demonstrating that a nonspecific monoclonal IgM (against influenza hemagglutinin), when coupled to the filters under identical conditions, had no effect on removal of HBsAg from serum. Most importantly, the monoclonal anti-HBsAg IgM-coupled filters quantitatively removed low levels of HBsAg from serum; after the third pass through the filter, HBsAg was undetectable in the perfusate. Further, the stability of the covalent bond between the anti-HBsAg IgM and regenerated cellulose acetate was shown by the lack of detectable murine monoclonal anti-HBsAg IgM in filtered serum despite 50 passages through the filter. Thus, we have demonstrated that monoclonal IgM antibodies with pre-defined specificity, when coupled to a biocompatible solid-phase support, may serve as a high-affinity and specific immunoabsorbant for quantitative removal and recovery of viral antigens from human serum. By using this approach, specific removal and recovery of many other substances from serum or plasma would seem possible.

Original languageEnglish (US)
Pages (from-to)3821-3825
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume80
Issue number12 I
StatePublished - 1983
Externally publishedYes

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Hepatitis B Antigens
Viral Antigens
Hepatitis B Surface Antigens
Immunoglobulin M
Serum
Cyanogen Bromide
Hemagglutinins
Cellulose
Human Influenza
Epitopes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Quantitative removal of hepatitis B viral antigens from serum by a monoclonal IgM coupled to a biocompatible solid-phase support. / Marciniak, Robert A; Wands, J. R.; Bruns, R. R.; Malchesky, P. S.; Nose, Y.; Haber, E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, No. 12 I, 1983, p. 3821-3825.

Research output: Contribution to journalArticle

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AU - Marciniak, Robert A

AU - Wands, J. R.

AU - Bruns, R. R.

AU - Malchesky, P. S.

AU - Nose, Y.

AU - Haber, E.

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