Quantitative proteomic analysis of Myc oncoprotein function

Yuzuru Shiio, Sam Donohoe, Eugene C. Yi, David R. Goodlett, Ruedi Aebersold, Robert N. Eisenman

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

This study applies a new quantitative proteomics technology to the analysis of the function of the Myc oncoprotein in mammalian cells. Employing isotope-coded affinity tag (ICAT™) reagent labeling and tandem mass spectrometry, the global pattern of protein expression in rat myc-null cells was compared with that of myc-plus cells (myc-null cells in which myc has been introduced) to generate a differential protein expression catalog. Expression differences among many functionally related proteins were identified, including reduction of proteases, induction of protein synthesis pathways and upregulation of anabolic enzymes in myc-plus cells, which are predicted to lead to increased cell mass (cell growth). In addition, reduction in the levels of adhesion molecules, actin network proteins and Rho pathway proteins were observed in myc-plus cells, leading to reduced focal adhesions and actin stress fibers as well as altered morphology. These effects are dependent on the highly conserved Myc Box II region. Our results reveal a novel cytoskeletal function for Myc and indicate the feasibility of quantitative whole-proteome analysis in mammalian cells.

Original languageEnglish (US)
Pages (from-to)5088-5096
Number of pages9
JournalEMBO Journal
Volume21
Issue number19
DOIs
StatePublished - Oct 1 2002
Externally publishedYes

Fingerprint

Oncogene Proteins
Proteomics
Proteins
Null Lymphocytes
Actins
Adhesion
Cells
Affinity Labels
Cell growth
Stress Fibers
Proteome
Focal Adhesions
Isotopes
Labeling
Mass spectrometry
Tandem Mass Spectrometry
Rats
Peptide Hydrolases
Up-Regulation
Molecules

Keywords

  • Cell growth
  • Cytoskeleton
  • ICAT™ reagent
  • Myc oncoprotein
  • Proteomics

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Shiio, Y., Donohoe, S., Yi, E. C., Goodlett, D. R., Aebersold, R., & Eisenman, R. N. (2002). Quantitative proteomic analysis of Myc oncoprotein function. EMBO Journal, 21(19), 5088-5096. https://doi.org/10.1093/emboj/cdf525

Quantitative proteomic analysis of Myc oncoprotein function. / Shiio, Yuzuru; Donohoe, Sam; Yi, Eugene C.; Goodlett, David R.; Aebersold, Ruedi; Eisenman, Robert N.

In: EMBO Journal, Vol. 21, No. 19, 01.10.2002, p. 5088-5096.

Research output: Contribution to journalArticle

Shiio, Y, Donohoe, S, Yi, EC, Goodlett, DR, Aebersold, R & Eisenman, RN 2002, 'Quantitative proteomic analysis of Myc oncoprotein function', EMBO Journal, vol. 21, no. 19, pp. 5088-5096. https://doi.org/10.1093/emboj/cdf525
Shiio Y, Donohoe S, Yi EC, Goodlett DR, Aebersold R, Eisenman RN. Quantitative proteomic analysis of Myc oncoprotein function. EMBO Journal. 2002 Oct 1;21(19):5088-5096. https://doi.org/10.1093/emboj/cdf525
Shiio, Yuzuru ; Donohoe, Sam ; Yi, Eugene C. ; Goodlett, David R. ; Aebersold, Ruedi ; Eisenman, Robert N. / Quantitative proteomic analysis of Myc oncoprotein function. In: EMBO Journal. 2002 ; Vol. 21, No. 19. pp. 5088-5096.
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