Quantitative proteomic analysis of Myc oncoprotein function

Yuzuru Shiio, Sam Donohoe, Eugene C. Yi, David R. Goodlett, Ruedi Aebersold, Robert N. Eisenman

Research output: Contribution to journalArticlepeer-review

174 Scopus citations


This study applies a new quantitative proteomics technology to the analysis of the function of the Myc oncoprotein in mammalian cells. Employing isotope-coded affinity tag (ICAT™) reagent labeling and tandem mass spectrometry, the global pattern of protein expression in rat myc-null cells was compared with that of myc-plus cells (myc-null cells in which myc has been introduced) to generate a differential protein expression catalog. Expression differences among many functionally related proteins were identified, including reduction of proteases, induction of protein synthesis pathways and upregulation of anabolic enzymes in myc-plus cells, which are predicted to lead to increased cell mass (cell growth). In addition, reduction in the levels of adhesion molecules, actin network proteins and Rho pathway proteins were observed in myc-plus cells, leading to reduced focal adhesions and actin stress fibers as well as altered morphology. These effects are dependent on the highly conserved Myc Box II region. Our results reveal a novel cytoskeletal function for Myc and indicate the feasibility of quantitative whole-proteome analysis in mammalian cells.

Original languageEnglish (US)
Pages (from-to)5088-5096
Number of pages9
JournalEMBO Journal
Issue number19
StatePublished - Oct 1 2002
Externally publishedYes


  • Cell growth
  • Cytoskeleton
  • ICAT™ reagent
  • Myc oncoprotein
  • Proteomics

ASJC Scopus subject areas

  • General Immunology and Microbiology
  • General Biochemistry, Genetics and Molecular Biology
  • Molecular Biology
  • General Neuroscience


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