Quantitative proteomic analysis of chromatin-associated factors

Yuzuru Shiio, Robert N. Eisenman, Eugene C. Yi, Sam Donohoe, David R. Goodlett, Ruedi Aebersold

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

A method to identify and quantify chromatin-associated proteins has been developed and applied to the analysis of changes in chromatin-associated proteins induced by Myc oncoprotein expression in human B lymphocytes. Chromatin-enriched fractions were isolated by differential detergent/salt extraction and analyzed by ICAT reagent labeling, multi-dimensional chromatography and tandem mass spectrometry. Many known chromatin-associated regulatory factors were identified and quantified. The method will be widely applicable to various biological systems and reveal changes in chromatin-associated regulatory factors that underlie biological phenomena.

Original languageEnglish (US)
Pages (from-to)696-703
Number of pages8
JournalJournal of the American Society for Mass Spectrometry
Volume14
Issue number7
DOIs
StatePublished - Jul 2003

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

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