Quantitative proteomic analysis of chromatin-associated factors

Yuzuru Shiio; Robert N. Eisenman; Eugene C. Yi; Sam Donohoe; David R. Goodlett; Ruedi Aebersold

doi:10.1016/S1044-0305(03)00204-6

Quantitative proteomic analysis of chromatin-associated factors

Yuzuru Shiio, Robert N. Eisenman, Eugene C. Yi, Sam Donohoe, David R. Goodlett, Ruedi Aebersold

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

A method to identify and quantify chromatin-associated proteins has been developed and applied to the analysis of changes in chromatin-associated proteins induced by Myc oncoprotein expression in human B lymphocytes. Chromatin-enriched fractions were isolated by differential detergent/salt extraction and analyzed by ICAT reagent labeling, multi-dimensional chromatography and tandem mass spectrometry. Many known chromatin-associated regulatory factors were identified and quantified. The method will be widely applicable to various biological systems and reveal changes in chromatin-associated regulatory factors that underlie biological phenomena.

Original language	English (US)
Pages (from-to)	696-703
Number of pages	8
Journal	Journal of the American Society for Mass Spectrometry
Volume	14
Issue number	7
DOIs	https://doi.org/10.1016/S1044-0305(03)00204-6
State	Published - Jul 2003
Externally published	Yes

ASJC Scopus subject areas

Structural Biology
Spectroscopy

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10.1016/S1044-0305(03)00204-6

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@article{c592db275b8647479a8876a962f4c12b,

title = "Quantitative proteomic analysis of chromatin-associated factors",

abstract = "A method to identify and quantify chromatin-associated proteins has been developed and applied to the analysis of changes in chromatin-associated proteins induced by Myc oncoprotein expression in human B lymphocytes. Chromatin-enriched fractions were isolated by differential detergent/salt extraction and analyzed by ICAT reagent labeling, multi-dimensional chromatography and tandem mass spectrometry. Many known chromatin-associated regulatory factors were identified and quantified. The method will be widely applicable to various biological systems and reveal changes in chromatin-associated regulatory factors that underlie biological phenomena.",

author = "Yuzuru Shiio and Eisenman, {Robert N.} and Yi, {Eugene C.} and Sam Donohoe and Goodlett, {David R.} and Ruedi Aebersold",

note = "Funding Information: The authors thank Dr. Dirk Eick for P493-6 cell line, Dr. Yoshiro Maru for anti-MAPK antibody, Dr. Yoshihiro Yoneda for anti-NIFK antibody, and Dr. Bruce Stillman for anti-RbAp48 antibody. This work was supported by grants from NIH (DRG, RA, RNE), by Federal Fund from the National Heart, Blood and Lung Institute, by NIH under contract N01-HV-28179, by a grant from Oxford Glycosciences, by the Interdisciplinary Research Training Fellowship from the Fred Hutchinson Cancer Research Center (YS), and by a gift from Merck and Co. to the Institute for Systems Biology. RNE is a research professor of the American Cancer Society.",

year = "2003",

month = jul,

doi = "10.1016/S1044-0305(03)00204-6",

language = "English (US)",

volume = "14",

pages = "696--703",

journal = "Journal of the American Society for Mass Spectrometry",

issn = "1044-0305",

publisher = "Springer New York",

number = "7",

}

TY - JOUR

T1 - Quantitative proteomic analysis of chromatin-associated factors

AU - Shiio, Yuzuru

AU - Eisenman, Robert N.

AU - Yi, Eugene C.

AU - Donohoe, Sam

AU - Goodlett, David R.

AU - Aebersold, Ruedi

N1 - Funding Information: The authors thank Dr. Dirk Eick for P493-6 cell line, Dr. Yoshiro Maru for anti-MAPK antibody, Dr. Yoshihiro Yoneda for anti-NIFK antibody, and Dr. Bruce Stillman for anti-RbAp48 antibody. This work was supported by grants from NIH (DRG, RA, RNE), by Federal Fund from the National Heart, Blood and Lung Institute, by NIH under contract N01-HV-28179, by a grant from Oxford Glycosciences, by the Interdisciplinary Research Training Fellowship from the Fred Hutchinson Cancer Research Center (YS), and by a gift from Merck and Co. to the Institute for Systems Biology. RNE is a research professor of the American Cancer Society.

PY - 2003/7

Y1 - 2003/7

N2 - A method to identify and quantify chromatin-associated proteins has been developed and applied to the analysis of changes in chromatin-associated proteins induced by Myc oncoprotein expression in human B lymphocytes. Chromatin-enriched fractions were isolated by differential detergent/salt extraction and analyzed by ICAT reagent labeling, multi-dimensional chromatography and tandem mass spectrometry. Many known chromatin-associated regulatory factors were identified and quantified. The method will be widely applicable to various biological systems and reveal changes in chromatin-associated regulatory factors that underlie biological phenomena.

AB - A method to identify and quantify chromatin-associated proteins has been developed and applied to the analysis of changes in chromatin-associated proteins induced by Myc oncoprotein expression in human B lymphocytes. Chromatin-enriched fractions were isolated by differential detergent/salt extraction and analyzed by ICAT reagent labeling, multi-dimensional chromatography and tandem mass spectrometry. Many known chromatin-associated regulatory factors were identified and quantified. The method will be widely applicable to various biological systems and reveal changes in chromatin-associated regulatory factors that underlie biological phenomena.

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U2 - 10.1016/S1044-0305(03)00204-6

DO - 10.1016/S1044-0305(03)00204-6

M3 - Article

C2 - 12837591

AN - SCOPUS:0038261923

VL - 14

SP - 696

EP - 703

JO - Journal of the American Society for Mass Spectrometry

JF - Journal of the American Society for Mass Spectrometry

SN - 1044-0305

IS - 7

ER -

Quantitative proteomic analysis of chromatin-associated factors

Abstract

ASJC Scopus subject areas

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