@article{c592db275b8647479a8876a962f4c12b,
title = "Quantitative proteomic analysis of chromatin-associated factors",
abstract = "A method to identify and quantify chromatin-associated proteins has been developed and applied to the analysis of changes in chromatin-associated proteins induced by Myc oncoprotein expression in human B lymphocytes. Chromatin-enriched fractions were isolated by differential detergent/salt extraction and analyzed by ICAT reagent labeling, multi-dimensional chromatography and tandem mass spectrometry. Many known chromatin-associated regulatory factors were identified and quantified. The method will be widely applicable to various biological systems and reveal changes in chromatin-associated regulatory factors that underlie biological phenomena.",
author = "Yuzuru Shiio and Eisenman, {Robert N.} and Yi, {Eugene C.} and Sam Donohoe and Goodlett, {David R.} and Ruedi Aebersold",
note = "Funding Information: The authors thank Dr. Dirk Eick for P493-6 cell line, Dr. Yoshiro Maru for anti-MAPK antibody, Dr. Yoshihiro Yoneda for anti-NIFK antibody, and Dr. Bruce Stillman for anti-RbAp48 antibody. This work was supported by grants from NIH (DRG, RA, RNE), by Federal Fund from the National Heart, Blood and Lung Institute, by NIH under contract N01-HV-28179, by a grant from Oxford Glycosciences, by the Interdisciplinary Research Training Fellowship from the Fred Hutchinson Cancer Research Center (YS), and by a gift from Merck and Co. to the Institute for Systems Biology. RNE is a research professor of the American Cancer Society.",
year = "2003",
month = jul,
doi = "10.1016/S1044-0305(03)00204-6",
language = "English (US)",
volume = "14",
pages = "696--703",
journal = "Journal of the American Society for Mass Spectrometry",
issn = "1044-0305",
publisher = "Springer New York",
number = "7",
}