Quantitative genetic analysis of glucose transporter 4 mRNA levels in baboon adipose

M. Elizabeth Tejero; J. Michael Proffitt; Shelley A. Cole; Jeanne H. Freeland-Graves; Guowen Cai; Kyle W. Peebles; Laura A. Cox; Michael C. Mahaney; Jeffrey Rogers; John L. VandeBerg; John Blangero; Anthony G. Comuzzie

doi:10.1038/oby.2004.205

Quantitative genetic analysis of glucose transporter 4 mRNA levels in baboon adipose

M. Elizabeth Tejero, J. Michael Proffitt, Shelley A. Cole, Jeanne H. Freeland-Graves, Guowen Cai, Kyle W. Peebles, Laura A. Cox, Michael C. Mahaney, Jeffrey Rogers, John L. VandeBerg, John Blangero, Anthony G. Comuzzie

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Objective: Glucose transporter 4 (GLUT4) is an insulin-responsive glucose transporter expressed in adipose tissue. A decrease of the mRNA abundance of GLUT4 in adipose tissue has been observed in conditions of insulin resistance. The objective was to conduct quantitative genetic analyses using GLUT4 mRNA levels in omental adipose tissue of baboons as a novel phenotype. Research Methods and Procedures: A blood sample and a biopsy of omental adipose tissue were collected from 418 adult, pedigreed baboons. Total RNA was isolated from adipose tissue biopsies, and GLUT4 mRNA abundance was assayed by quantitative, real-time reverse transcription-polymerase chain reaction. Insulin and glucose were determined in fasting plasma by standard methods. Quantitative genetic analyses were conducted using GLUT4 mRNA, insulin, and glucose as quantitative traits. Results: GLUT4 mRNA expression in omental adipose tissue was heritable (h² = 0.23, p = 0.001). Bivariate genetic analyses revealed a significant genetic correlation (ρ_G) between GLUT4 mRNA abundance and both body weight (ρ_G = 0.63, p = 0.007), BMI (ρ_G = 0.59, p = 0.02) and insulin (ρ_G = 0.72, p = 0.04). A genome scan was conducted, and a quantitative trait locus was detected on chromosome 10p12 with a logarithm of the odds ratio score of 1.1. Discussion: GLUT4 mRNA abundance in omental adipose tissue has a significant genetic component. These findings suggest that expression of GLUT4 mRNA, plasma insulin levels, and body weight may be regulated by common genes.

Original language	English (US)
Pages (from-to)	1652-1657
Number of pages	6
Journal	Obesity Research
Volume	12
Issue number	10
DOIs	https://doi.org/10.1038/oby.2004.205
State	Published - 2004
Externally published	Yes

Keywords

Adipose tissue
Animal model
Gene expression
Genetic analysis
Insulin resistance

ASJC Scopus subject areas

Medicine (miscellaneous)
Food Science
Endocrinology, Diabetes and Metabolism
Endocrinology
Public Health, Environmental and Occupational Health

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10.1038/oby.2004.205

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@article{a5aa0708e25e40f3af58b88b474b0126,

title = "Quantitative genetic analysis of glucose transporter 4 mRNA levels in baboon adipose",

abstract = "Objective: Glucose transporter 4 (GLUT4) is an insulin-responsive glucose transporter expressed in adipose tissue. A decrease of the mRNA abundance of GLUT4 in adipose tissue has been observed in conditions of insulin resistance. The objective was to conduct quantitative genetic analyses using GLUT4 mRNA levels in omental adipose tissue of baboons as a novel phenotype. Research Methods and Procedures: A blood sample and a biopsy of omental adipose tissue were collected from 418 adult, pedigreed baboons. Total RNA was isolated from adipose tissue biopsies, and GLUT4 mRNA abundance was assayed by quantitative, real-time reverse transcription-polymerase chain reaction. Insulin and glucose were determined in fasting plasma by standard methods. Quantitative genetic analyses were conducted using GLUT4 mRNA, insulin, and glucose as quantitative traits. Results: GLUT4 mRNA expression in omental adipose tissue was heritable (h2 = 0.23, p = 0.001). Bivariate genetic analyses revealed a significant genetic correlation (ρG) between GLUT4 mRNA abundance and both body weight (ρG = 0.63, p = 0.007), BMI (ρG = 0.59, p = 0.02) and insulin (ρG = 0.72, p = 0.04). A genome scan was conducted, and a quantitative trait locus was detected on chromosome 10p12 with a logarithm of the odds ratio score of 1.1. Discussion: GLUT4 mRNA abundance in omental adipose tissue has a significant genetic component. These findings suggest that expression of GLUT4 mRNA, plasma insulin levels, and body weight may be regulated by common genes.",

keywords = "Adipose tissue, Animal model, Gene expression, Genetic analysis, Insulin resistance",

author = "Tejero, {M. Elizabeth} and Proffitt, {J. Michael} and Cole, {Shelley A.} and Freeland-Graves, {Jeanne H.} and Guowen Cai and Peebles, {Kyle W.} and Cox, {Laura A.} and Mahaney, {Michael C.} and Jeffrey Rogers and VandeBerg, {John L.} and John Blangero and Comuzzie, {Anthony G.}",

year = "2004",

doi = "10.1038/oby.2004.205",

language = "English (US)",

volume = "12",

pages = "1652--1657",

journal = "Obesity",

issn = "1930-7381",

publisher = "Wiley-Blackwell",

number = "10",

}

TY - JOUR

T1 - Quantitative genetic analysis of glucose transporter 4 mRNA levels in baboon adipose

AU - Tejero, M. Elizabeth

AU - Proffitt, J. Michael

AU - Cole, Shelley A.

AU - Freeland-Graves, Jeanne H.

AU - Cai, Guowen

AU - Peebles, Kyle W.

AU - Cox, Laura A.

AU - Mahaney, Michael C.

AU - Rogers, Jeffrey

AU - VandeBerg, John L.

AU - Blangero, John

AU - Comuzzie, Anthony G.

PY - 2004

Y1 - 2004

N2 - Objective: Glucose transporter 4 (GLUT4) is an insulin-responsive glucose transporter expressed in adipose tissue. A decrease of the mRNA abundance of GLUT4 in adipose tissue has been observed in conditions of insulin resistance. The objective was to conduct quantitative genetic analyses using GLUT4 mRNA levels in omental adipose tissue of baboons as a novel phenotype. Research Methods and Procedures: A blood sample and a biopsy of omental adipose tissue were collected from 418 adult, pedigreed baboons. Total RNA was isolated from adipose tissue biopsies, and GLUT4 mRNA abundance was assayed by quantitative, real-time reverse transcription-polymerase chain reaction. Insulin and glucose were determined in fasting plasma by standard methods. Quantitative genetic analyses were conducted using GLUT4 mRNA, insulin, and glucose as quantitative traits. Results: GLUT4 mRNA expression in omental adipose tissue was heritable (h2 = 0.23, p = 0.001). Bivariate genetic analyses revealed a significant genetic correlation (ρG) between GLUT4 mRNA abundance and both body weight (ρG = 0.63, p = 0.007), BMI (ρG = 0.59, p = 0.02) and insulin (ρG = 0.72, p = 0.04). A genome scan was conducted, and a quantitative trait locus was detected on chromosome 10p12 with a logarithm of the odds ratio score of 1.1. Discussion: GLUT4 mRNA abundance in omental adipose tissue has a significant genetic component. These findings suggest that expression of GLUT4 mRNA, plasma insulin levels, and body weight may be regulated by common genes.

AB - Objective: Glucose transporter 4 (GLUT4) is an insulin-responsive glucose transporter expressed in adipose tissue. A decrease of the mRNA abundance of GLUT4 in adipose tissue has been observed in conditions of insulin resistance. The objective was to conduct quantitative genetic analyses using GLUT4 mRNA levels in omental adipose tissue of baboons as a novel phenotype. Research Methods and Procedures: A blood sample and a biopsy of omental adipose tissue were collected from 418 adult, pedigreed baboons. Total RNA was isolated from adipose tissue biopsies, and GLUT4 mRNA abundance was assayed by quantitative, real-time reverse transcription-polymerase chain reaction. Insulin and glucose were determined in fasting plasma by standard methods. Quantitative genetic analyses were conducted using GLUT4 mRNA, insulin, and glucose as quantitative traits. Results: GLUT4 mRNA expression in omental adipose tissue was heritable (h2 = 0.23, p = 0.001). Bivariate genetic analyses revealed a significant genetic correlation (ρG) between GLUT4 mRNA abundance and both body weight (ρG = 0.63, p = 0.007), BMI (ρG = 0.59, p = 0.02) and insulin (ρG = 0.72, p = 0.04). A genome scan was conducted, and a quantitative trait locus was detected on chromosome 10p12 with a logarithm of the odds ratio score of 1.1. Discussion: GLUT4 mRNA abundance in omental adipose tissue has a significant genetic component. These findings suggest that expression of GLUT4 mRNA, plasma insulin levels, and body weight may be regulated by common genes.

KW - Adipose tissue

KW - Animal model

KW - Gene expression

KW - Genetic analysis

KW - Insulin resistance

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DO - 10.1038/oby.2004.205

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VL - 12

SP - 1652

EP - 1657

JO - Obesity

JF - Obesity

IS - 10

ER -

Quantitative genetic analysis of glucose transporter 4 mRNA levels in baboon adipose

Abstract

Keywords

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