Quantitative autoradiography of serotonin uptake sites in rat brain using [3H]cyanoimipramine

Gyula B. Kovachich, Carl E. Aronson, David J. Brunswick, Alan Frazer

Research output: Contribution to journalArticle

89 Scopus citations

Abstract

The binding of [3H]cyanoimipramine to serotonin uptake sites in rat brain slices was studied using quantitative autoradiography. Binding was of high affinity and was to a single class of binding site. This is in contrast to results previously obtained by others with [3H]imipramine where two binding sites were observed. The sites labeled by [3H]cyanoimipramine had properties consistent with this ligand labeling serotonin uptake sites, as: (1) binding is displaced by drugs which are potent inhibitors of serotonin uptake but not by drugs which are weak inhibitors of uptake; (2) binding is dependent on the presence of sodium ions as is the uptake of serotonin; (3) binding is almost completely eliminated in the brains of rats lesioned by the serotonin neurotoxin 5,7-dihydroxytryptamine; (4) the distribution of binding sites throughout the rat brain is highly correlated with that found previously for [3H]indalpine, a potent serotonin uptake inhibitor, and for [3H]imipramine. The properties of binding of [3H]cyanoimipramine make it an ideal ligand for the quantitative autoradiography of serotonin uptake sites.

Original languageEnglish (US)
Pages (from-to)78-88
Number of pages11
JournalBrain Research
Volume454
Issue number1-2
DOIs
StatePublished - Jun 28 1988
Externally publishedYes

Keywords

  • Autoradiography, quantitative
  • Cyanoimipramine
  • Serotonin uptake site

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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