Quantitative analysis of two opioid peptides in plasma by liquid chromatography-electrospray ionization tandem mass spectrometry

Cristina D. Márquez, Susan T. Weintraub, Philip C. Smith

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Quantitative analysis of two opioid peptides, DSLET [(D-Ser2)Leu-enkephalin-Thr6] and Met-enkephalin-Arg-Gly-Leu, was performed using microbore liquid chromatography interfaced to electrospray ionization tandem mass spectrometry. Validation of the methodology was demonstrated for each peptide in plasma. Quantitative analyses were performed through the use of a deuterium labelled peptide analog as an internal standard. Linearity was observed for the analysis of DSLET (5-1000 ng/ml) and Met-enkephalin-Arg-Gly-Leu (1-1000 ng/ml) in plasma with a limit of detection of 0.25 ng/ml for Met-enkephalin-Arg-Gly-Leu and 1.0 ng/ml for DSLET. In general, the observed concentrations showed good reproducibility with coefficients of variation of within 15%. In the concentration range studied, only 0.5 ml of plasma was required for optimal detection of Met-enkephalin-Arg-Gly-Leu and 0.25 ml for DSLET. Application of this method was demonstrated by studying the disposition of DSLET in a rat. DSLET administered to a rat exhibited a short half-life and a high clearance value.

Original languageEnglish (US)
Pages (from-to)21-30
Number of pages10
JournalJournal of Chromatography B: Biomedical Applications
Volume694
Issue number1
DOIs
StatePublished - Jun 20 1997

Keywords

  • (D-Ser)Leu-enkaphalin-Thr
  • Met-enkaphalin-Arg-Gly-Leu
  • Opioid peptides

ASJC Scopus subject areas

  • Chemistry(all)

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