Quantitative analysis of hydralazine pyruvic acid hydrazone, the major plasma metabolite of hydralazine

Klaus D. Haegele; Harold B. Skrdlant; Tore Talseth; John L. McNay; Alexander M.M. Shepherd; William A. Clementi

doi:10.1016/S0021-9673(00)87883-8

Quantitative analysis of hydralazine pyruvic acid hydrazone, the major plasma metabolite of hydralazine

Klaus D. Haegele
, Harold B. Skrdlant
, Tore Talseth
, John L. McNay
, Alexander M.M. Shepherd
, William A. Clementi

Division of General Medicine

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A specific, high-performance liquid chromatographic techinique for the measurement of hydralazine pyruvic acid hydrazone is described. This method utilized reversed-phase chromatography for the separation of this hydrophilic metabolite of hydralazine from other fluid constituents present in serum, plasma, or urine of human volunteers and rabbits receiving hydralazine. Detection of the compound of interest is accomplished spectrophotometrically at 250 nm.

Original language	English (US)
Pages (from-to)	171-179
Number of pages	9
Journal	Journal of Chromatography A
Volume	187
Issue number	1
DOIs	https://doi.org/10.1016/S0021-9673(00)87883-8
State	Published - Jan 4 1980

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/S0021-9673(00)87883-8

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@article{d75d420a04b1463d96557d4010444b79,

title = "Quantitative analysis of hydralazine pyruvic acid hydrazone, the major plasma metabolite of hydralazine",

abstract = "A specific, high-performance liquid chromatographic techinique for the measurement of hydralazine pyruvic acid hydrazone is described. This method utilized reversed-phase chromatography for the separation of this hydrophilic metabolite of hydralazine from other fluid constituents present in serum, plasma, or urine of human volunteers and rabbits receiving hydralazine. Detection of the compound of interest is accomplished spectrophotometrically at 250 nm.",

author = "Haegele, \{Klaus D.\} and Skrdlant, \{Harold B.\} and Tore Talseth and McNay, \{John L.\} and Shepherd, \{Alexander M.M.\} and Clementi, \{William A.\}",

note = "Funding Information: This work was supportedb y NIH/NXGM!S grant No. GM 24092, the Veterans Admin.\&\&on, NIH/NIGMS grant No. GM 07014 and an American Heart Association, Texas AfEliate grant. Tore Taketh is a recipient of a Merck, Sharp \& Dohme LntemationalF eUowsEipi n Clinical Phatmacol ogy. T\&e techniczLals sk3ta.n\textasciitilde{} ofM rs. Susan B. Ktiaad Ms. Kay BaU\&er, as welt as the semeta\&I heIp ofI !\&. BarbaraB oyle and Ms. Diana Resemiezi s most grat\&Wy appreciated.",

year = "1980",

month = jan,

day = "4",

doi = "10.1016/S0021-9673(00)87883-8",

language = "English (US)",

volume = "187",

pages = "171--179",

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TY - JOUR

T1 - Quantitative analysis of hydralazine pyruvic acid hydrazone, the major plasma metabolite of hydralazine

AU - Haegele, Klaus D.

AU - Skrdlant, Harold B.

AU - Talseth, Tore

AU - McNay, John L.

AU - Shepherd, Alexander M.M.

AU - Clementi, William A.

N1 - Funding Information: This work was supportedb y NIH/NXGM!S grant No. GM 24092, the Veterans Admin.&&on, NIH/NIGMS grant No. GM 07014 and an American Heart Association, Texas AfEliate grant. Tore Taketh is a recipient of a Merck, Sharp & Dohme LntemationalF eUowsEipi n Clinical Phatmacol ogy. T&e techniczLals sk3ta.n~ ofM rs. Susan B. Ktiaad Ms. Kay BaU&er, as welt as the semeta&I heIp ofI !&. BarbaraB oyle and Ms. Diana Resemiezi s most grat&Wy appreciated.

PY - 1980/1/4

Y1 - 1980/1/4

N2 - A specific, high-performance liquid chromatographic techinique for the measurement of hydralazine pyruvic acid hydrazone is described. This method utilized reversed-phase chromatography for the separation of this hydrophilic metabolite of hydralazine from other fluid constituents present in serum, plasma, or urine of human volunteers and rabbits receiving hydralazine. Detection of the compound of interest is accomplished spectrophotometrically at 250 nm.

AB - A specific, high-performance liquid chromatographic techinique for the measurement of hydralazine pyruvic acid hydrazone is described. This method utilized reversed-phase chromatography for the separation of this hydrophilic metabolite of hydralazine from other fluid constituents present in serum, plasma, or urine of human volunteers and rabbits receiving hydralazine. Detection of the compound of interest is accomplished spectrophotometrically at 250 nm.

UR - https://www.scopus.com/pages/publications/0018865165

UR - https://www.scopus.com/pages/publications/0018865165#tab=citedBy

U2 - 10.1016/S0021-9673(00)87883-8

DO - 10.1016/S0021-9673(00)87883-8

M3 - Article

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AN - SCOPUS:0018865165

SN - 0021-9673

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JF - Journal of Chromatography A

IS - 1

ER -

Quantitative analysis of hydralazine pyruvic acid hydrazone, the major plasma metabolite of hydralazine

Abstract

ASJC Scopus subject areas

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