Quantitative analysis of hydralazine pyruvic acid hydrazone, the major plasma metabolite of hydralazine

Klaus D. Haegele, Harold B. Skrdlant, Tore Talseth, John L. McNay, Alexander M.M. Shepherd, William A. Clementi

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

A specific, high-performance liquid chromatographic techinique for the measurement of hydralazine pyruvic acid hydrazone is described. This method utilized reversed-phase chromatography for the separation of this hydrophilic metabolite of hydralazine from other fluid constituents present in serum, plasma, or urine of human volunteers and rabbits receiving hydralazine. Detection of the compound of interest is accomplished spectrophotometrically at 250 nm.

Original languageEnglish (US)
Pages (from-to)171-179
Number of pages9
JournalJournal of Chromatography A
Volume187
Issue number1
DOIs
StatePublished - Jan 4 1980

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Haegele, K. D., Skrdlant, H. B., Talseth, T., McNay, J. L., Shepherd, A. M. M., & Clementi, W. A. (1980). Quantitative analysis of hydralazine pyruvic acid hydrazone, the major plasma metabolite of hydralazine. Journal of Chromatography A, 187(1), 171-179. https://doi.org/10.1016/S0021-9673(00)87883-8