Quantitative analysis of creatinine in urine by metalized nanostructured parylene

Hui Wang, Niranjan Malvadkar, S. Koytek, J. Bylander, W. Brian Reeves, Melik C. Demirel

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


A highly accurate, real-time multisensor agent monitor for biomarker detection is required for early detection of kidney diseases. Urine creatinine level can provide useful information on the status of the kidney. We prepare nanostructured surface-enhanced Raman spectroscopy (SERS) substrates without template or lithography, which provides controllable, well-organized nanostructures on the surface, for the quantitative analysis of creatinine concentration in urine. We present our work on sensitivity of the SERS substrate to urine samples collected from diabetic patients and healthy persons. We report the preparation of a new type of SERS substrate, which provides fast (<10s), highly sensitive (creatinine concentration <0.5 μg/mL) and reproducible (<5% variation) detection of urine. Our method to analyze the creatinine level in urine is in good agreement with the enzymatic method.

Original languageEnglish (US)
Article number027004
JournalJournal of biomedical optics
Issue number2
StatePublished - 2010
Externally publishedYes


  • Creatinine
  • Nanostructured poly(chloro-p-xylylene)
  • Raman
  • Surface-enhanced Raman spectroscopy
  • Urine

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering
  • Biomaterials


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