@article{699e8b4016ba46829c2bb7c33b429227,
title = "Quantification of DNA double-strand breaks using Geant4-DNA",
abstract = "Purpose: This study aims to standardize the simulation procedure in measuring DNA double-strand breaks (DSBs), by using advanced Monte Carlo toolkits, and newly introduced experimental methods for DNA DSB measurement. Methods: For the experimental quantification of DNA DSB, an innovative DNA dosimeter was used to produce experimental data. GATE in combination with Geant4-DNA toolkit were exploited to simulate the experimental environment. The PDB4DNA example of Geant4-DNA was upgraded and investigated. Parameters of the simulation such energy threshold (ET) for a strand break and base pair threshold (BPT) for a DSB were evaluated, depending on the dose. Results: Simulations resulted to minimum differentiation in comparison to experimental data for ET = 19 ± 1 eV and BPT = 10 bp, and high differentiation for ET<17.5 eV or ET>22.5 eV and BPT = 10 bp. There was also small differentiation for ET = 17.5 eV and BPT = 6 bp. Uncertainty has been kept lower than 3%. Conclusions: This study includes first results on the quantification of DNA double-strand breaks. The energy spectrum of a LINAC was simulated and used for the first time to irradiate DNA molecules. Simulation outcome was validated on experimental data that were produced by a prototype DNA dosimeter.",
keywords = "DNA dosimetry, DNA double-strand breaks, Geant4-DNA, Monte Carlo simulations, radiobiology",
author = "Chatzipapas, {Konstantinos P.} and Panagiotis Papadimitroulas and Mohammad Obeidat and McConnell, {Kristen A.} and Neil Kirby and George Loudos and Niko Papanikolaou and Kagadis, {George C.}",
note = "Funding Information: This study is part of a project that has received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sk?odowska-Curie grant agreement No 691203. This work was co-supported by the European Union Seventh Framework Programme (FP7/2007-2013) under Grant 602621-TRIMAGE. Thanks to the Greek State Scholarship Foundation, ?Human Resources Development, Education and lifelong learning? (NSRF 2014-2020). An action entitled ?Scholarship Scheme?for postgraduate studies in second cycle?. This work was partly supported by computational time granted from the Greek Research & Technology Network (GRNET) in the National HPC facility ? ARIS ? under project ID pr005035_taskp-NuPeDose. This work was also supported in part by grants from the Cancer Prevention Research Institute of Texas (RP140105 and RP170345). Funding Information: This study is part of a project that has received funding from the European Union{\textquoteright}s Horizon 2020 research and innovation program under the Marie Sk{\l}odowska-Curie grant agreement No 691203. This work was co-supported by the European Union Seventh Framework Programme (FP7/2007-2013) under Grant 602621-TRIMAGE. Thanks to the Greek State Scholarship Foundation, “Human Resources Development, Education and lifelong learning” (NSRF 2014-2020). An action entitled “Scholarship Scheme for postgraduate studies in second cycle”. This work was partly supported by computational time granted from the Greek Research & Technology Network (GRNET) in the National HPC facility – ARIS – under project ID pr005035_taskp-NuPeDose. This work was also supported in part by grants from the Cancer Prevention Research Institute of Texas (RP140105 and RP170345). Publisher Copyright: {\textcopyright} 2018 The Authors. Medical Physics published by Wiley Periodicals, Inc. on behalf of American Association of Physicists in Medicine.",
year = "2019",
month = jan,
doi = "10.1002/mp.13290",
language = "English (US)",
volume = "46",
pages = "405--413",
journal = "Medical Physics",
issn = "0094-2405",
publisher = "AAPM - American Association of Physicists in Medicine",
number = "1",
}