Qualitative and quantitative separation of a series of phorbol-ester tumor promoters by high-pressure liquid chromatography

D. L. Berry, M. R. Lieber, S. M. Fischer, T. J. Slaga

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A high-pressure liquid chromatographic (HPLC) method using a micro-particulate silica column and gradient elution was developed that separated 12-O-tetradecanoylphorbol-13-acetate (TPA) from 20-oxo-TPA; 12-O-tetrade-canoylphorbol (TP);13-O-acetylphorbol (PA), and from the diterpene alcohol, phorbol (P). A series of other phorbol-ester tumor promoters were also separated via HPLC. Spectrophotometric determination at 232 nm allowed detection sensitivities of 0.05 μg of TPA. When tritiated TPA was applied to mouse skin, the majority of the tritiated product recovered was TPA, indicating only minimal metabolism of TPA and no need for metabolic activation for tumor promotion.

Original languageEnglish (US)
Pages (from-to)125-132
Number of pages8
JournalCancer Letters
Issue numberC
StatePublished - 1977


ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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