TY - JOUR
T1 - Pyrosequencing to detect mutations in FKS1 that confer reduced echinocandin susceptibility in Candida albicans
AU - Wiederhold, Nathan P.
AU - Grabinski, Jodi L.
AU - Garcia-Effron, Guillermo
AU - Perlin, David S.
AU - Lee, Samuel A.
PY - 2008/11
Y1 - 2008/11
N2 - Pyrosequencing was compared to Sanger dideoxy sequencing to detect mutations in FKS1 responsible for reduced echinocandin susceptibility in Candida albicans. These methods were in complete agreement for 10 of 12 clinical isolates with elevated echinocandin MICs, supporting the potential feasibility of pyrosequencing to detect mutations within diploid fungi.
AB - Pyrosequencing was compared to Sanger dideoxy sequencing to detect mutations in FKS1 responsible for reduced echinocandin susceptibility in Candida albicans. These methods were in complete agreement for 10 of 12 clinical isolates with elevated echinocandin MICs, supporting the potential feasibility of pyrosequencing to detect mutations within diploid fungi.
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U2 - 10.1128/AAC.00959-08
DO - 10.1128/AAC.00959-08
M3 - Article
C2 - 18794385
AN - SCOPUS:55849095115
VL - 52
SP - 4145
EP - 4148
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
SN - 0066-4804
IS - 11
ER -