Pyrosequencing to detect mutations in FKS1 that confer reduced echinocandin susceptibility in Candida albicans

Nathan P. Wiederhold, Jodi L. Grabinski, Guillermo Garcia-Effron, David S. Perlin, Samuel A. Lee

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Pyrosequencing was compared to Sanger dideoxy sequencing to detect mutations in FKS1 responsible for reduced echinocandin susceptibility in Candida albicans. These methods were in complete agreement for 10 of 12 clinical isolates with elevated echinocandin MICs, supporting the potential feasibility of pyrosequencing to detect mutations within diploid fungi.

Original languageEnglish (US)
Pages (from-to)4145-4148
Number of pages4
JournalAntimicrobial agents and chemotherapy
Volume52
Issue number11
DOIs
StatePublished - Nov 1 2008

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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