Pyrosequencing to detect mutations in FKS1 that confer reduced echinocandin susceptibility in Candida albicans

Nathan P. Wiederhold; Jodi L. Grabinski; Guillermo Garcia-Effron; David S. Perlin; Samuel A. Lee

doi:10.1128/AAC.00959-08

Pyrosequencing to detect mutations in FKS1 that confer reduced echinocandin susceptibility in Candida albicans

Nathan P. Wiederhold, Jodi L. Grabinski, Guillermo Garcia-Effron, David S. Perlin, Samuel A. Lee

Pathology

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Pyrosequencing was compared to Sanger dideoxy sequencing to detect mutations in FKS1 responsible for reduced echinocandin susceptibility in Candida albicans. These methods were in complete agreement for 10 of 12 clinical isolates with elevated echinocandin MICs, supporting the potential feasibility of pyrosequencing to detect mutations within diploid fungi.

Original language	English (US)
Pages (from-to)	4145-4148
Number of pages	4
Journal	Antimicrobial agents and chemotherapy
Volume	52
Issue number	11
DOIs	https://doi.org/10.1128/AAC.00959-08
State	Published - Nov 2008

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

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abstract = "Pyrosequencing was compared to Sanger dideoxy sequencing to detect mutations in FKS1 responsible for reduced echinocandin susceptibility in Candida albicans. These methods were in complete agreement for 10 of 12 clinical isolates with elevated echinocandin MICs, supporting the potential feasibility of pyrosequencing to detect mutations within diploid fungi.",

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