Purification of thiosulfate sulfurtransferase by selective immobilization on blue agarose

Paul M. Horowitz

doi:10.1016/0003-2697(78)90803-5

Purification of thiosulfate sulfurtransferase by selective immobilization on blue agarose

Paul M. Horowitz

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

A new method for isolating crystalline bovine liver thiosulfate sulfurtransferase has been developed which relies on the selective binding of the enzyme to agarose-immobilized Cibacron Blue F3GA. This preparation has the advantages of simplicity, reproducibility, and rapidity. It is also suggested that the introduction of a binding and elution step will materially aid previously published procedures.

Original language	English (US)
Pages (from-to)	751-753
Number of pages	3
Journal	Analytical Biochemistry
Volume	86
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(78)90803-5
State	Published - Jun 1 1978
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(78)90803-5

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abstract = "A new method for isolating crystalline bovine liver thiosulfate sulfurtransferase has been developed which relies on the selective binding of the enzyme to agarose-immobilized Cibacron Blue F3GA. This preparation has the advantages of simplicity, reproducibility, and rapidity. It is also suggested that the introduction of a binding and elution step will materially aid previously published procedures.",

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N2 - A new method for isolating crystalline bovine liver thiosulfate sulfurtransferase has been developed which relies on the selective binding of the enzyme to agarose-immobilized Cibacron Blue F3GA. This preparation has the advantages of simplicity, reproducibility, and rapidity. It is also suggested that the introduction of a binding and elution step will materially aid previously published procedures.

AB - A new method for isolating crystalline bovine liver thiosulfate sulfurtransferase has been developed which relies on the selective binding of the enzyme to agarose-immobilized Cibacron Blue F3GA. This preparation has the advantages of simplicity, reproducibility, and rapidity. It is also suggested that the introduction of a binding and elution step will materially aid previously published procedures.

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ER -

Purification of thiosulfate sulfurtransferase by selective immobilization on blue agarose

Abstract

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