Purification of norman murine sarcoma DNA polymerase alpha forms with different DNA template primer binding affinity and different specific activity

V. L. Sylvia, J. O. Norman, G. M. Curtin, J. Stec, D. L. Busbee

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

1. 1. DNA polymerase alpha was isolated from Norman Murine Myxosarcoma cells using ion exchange, immunoaffinity, and DNA affinity chromatography, showing two distinct enzyme forms designated A1 and A2. 2. 2. Chromatographic analysis of polymerase alpha forms A1 and A2 indicate a charge difference and a difference in affinity of binding to DNA between polymerase alpha forms which were equally reactive to anti-DNA polymerase alpha monoclonal IgG. 3. 3. Polymerase A1 specific activity was about 3600 U/mg while A2 specific activity was about 40,000 U/mg.

Original languageEnglish (US)
Pages (from-to)203-209
Number of pages7
JournalInternational Journal of Biochemistry
Volume21
Issue number2
DOIs
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

Fingerprint

Dive into the research topics of 'Purification of norman murine sarcoma DNA polymerase alpha forms with different DNA template primer binding affinity and different specific activity'. Together they form a unique fingerprint.

Cite this